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Pnf κb

Manufactured by Merck Group
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PNF-κB is a laboratory equipment product. It serves as a tool for the measurement and analysis of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway. The core function of PNF-κB is to provide researchers with the means to study the activation and regulation of this important transcription factor, which is involved in various cellular processes.

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Lab products found in correlation

Nf κb, by Merck Group (2 mentions) Pstat3, by Merck Group (2 mentions) α tubulin, by Merck Group (2 mentions) Pvdf membrane, by Merck Group (2 mentions) P jnk, by Merck Group (2 mentions) Pegfr, by Merck Group (2 mentions) Pikkα β, by Merck Group (2 mentions)

2 protocols using pnf κb

1

Comprehensive Protein Analysis Protocol

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Protein lysates were prepared according to standard protocols. Lysed protein was resuspended in denaturing protein-loading buffer. Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The following antibodies were used: pEGFR (Tyr1068 CST #3777; clone D7A5; 1/1000; Tyr1173 CST#4407; clone 53A5; 1/1000), EGFR (CST #4267; clone D38B1; 1/500), pJNK (CST #9255; clone G9; 1/2000), JNK (CST #9252; 1/1000), pp38 (CST #4631; clone 12F8; 1/1000), p38 (CST #9212; 1/1000), pIKKα/β (CST #2697; clone 16A6; 1/1000), IKKβ (CST #2370; clone 2C8; 1/1000), pNF-κB (CST #3033; clone 93H1; 1/1000), NF-κB (CST #3034; 1/1000), pStat3 (CST#9145; clone D3A7; 1/2000), Stat3 (sc-7179; 1/1000), Actin (Sigma A2066; 1/1000), α-Tubulin (Sigma T9026; clone DM1A; 1/500).
Lanaya H., Natarajan A., Komposch K., Li L., Amberg N., Chen L., Wculek S.K., Hammer M., Zenz R., Peck-Radosavljevic M., Sieghart W., Trauner M., Wang H, & Sibilia M. (2014). EGFR has a tumor-promoting role in liver macrophages during hepatocellular carcinoma formation. Nature cell biology, 16(10), 972-7.
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2

Comprehensive Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were prepared according to standard protocols. Lysed protein was resuspended in denaturing protein-loading buffer. Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The following antibodies were used: pEGFR (Tyr1068 CST #3777; clone D7A5; 1/1000; Tyr1173 CST#4407; clone 53A5; 1/1000), EGFR (CST #4267; clone D38B1; 1/500), pJNK (CST #9255; clone G9; 1/2000), JNK (CST #9252; 1/1000), pp38 (CST #4631; clone 12F8; 1/1000), p38 (CST #9212; 1/1000), pIKKα/β (CST #2697; clone 16A6; 1/1000), IKKβ (CST #2370; clone 2C8; 1/1000), pNF-κB (CST #3033; clone 93H1; 1/1000), NF-κB (CST #3034; 1/1000), pStat3 (CST#9145; clone D3A7; 1/2000), Stat3 (sc-7179; 1/1000), Actin (Sigma A2066; 1/1000), α-Tubulin (Sigma T9026; clone DM1A; 1/500).
Lanaya H., Natarajan A., Komposch K., Li L., Amberg N., Chen L., Wculek S.K., Hammer M., Zenz R., Peck-Radosavljevic M., Sieghart W., Trauner M., Wang H, & Sibilia M. (2014). EGFR has a tumor-promoting role in liver macrophages during hepatocellular carcinoma formation. Nature cell biology, 16(10), 972-7.
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