Kidneys were removed from both the control and UDC groups, and imaged with Carestream In Vivo MS Fx Pro (Carestream Health, USA) to capture X-ray and corresponding fluorescent images of the uterine cells that engrafted the kidney.
In vivo ms fx pro
Manufactured by Carestream
Sourced in United States
The In Vivo MS FX PRO is a multipurpose laboratory instrument designed for small animal imaging. It utilizes advanced optical and digital technologies to capture high-resolution images and perform non-invasive, real-time analysis of biological processes within living subjects.
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Lab products found in correlation
10 protocols using in vivo ms fx pro
1
Kidney Imaging of Uterine Cell Engraftment
2
Imaging Extracted Organs with X-ray and GFP
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After organs were extracted, they were imaged with Carestream In-Vivo MS Fx Pro (Carestream Health, USA) to capture X-ray and corresponding GFP fluorescent images.
3
Imaging Uterine GFP Fluorescence
4
Measuring IL-1β Tumor Fluorescence
5
Tumor-targeted Oligomer Localization
6
In Vivo Imaging of Photosensitizer Biodistribution
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4T1 tumor bearing
female BALB/c mice with an average tumor volume of 80 mm3 were divided into two groups and intravenously administered with
10 mg/kg of photosensitizer1 -PDT and 2 -PDT , respectively. Mice (n = 3 for each time point)55 (link) were then
sacrificed at different time points (0, 15 min, 1, 3, 6, 24, 48, and
72 h post compound administration), and major organs such as liver,
spleen, lung, kidney, lymph nodes, skin, eye, and tumor tissues were
harvested. Organs and tissues were imaged using an In Vivo MS FX PRO
(Carestream Molecular Imaging, Woodbridge, CT) with an excitation
filter at 530 nm and emission filter at 600 nm. Mice treated with
saline were used as control. Fluorescence intensities of each organ
and tissue were quantified using Carestream Molecular Imaging software
5.0 (Woodbridge, CT).
female BALB/c mice with an average tumor volume of 80 mm3 were divided into two groups and intravenously administered with
10 mg/kg of photosensitizer
sacrificed at different time points (0, 15 min, 1, 3, 6, 24, 48, and
72 h post compound administration), and major organs such as liver,
spleen, lung, kidney, lymph nodes, skin, eye, and tumor tissues were
harvested. Organs and tissues were imaged using an In Vivo MS FX PRO
(Carestream Molecular Imaging, Woodbridge, CT) with an excitation
filter at 530 nm and emission filter at 600 nm. Mice treated with
saline were used as control. Fluorescence intensities of each organ
and tissue were quantified using Carestream Molecular Imaging software
5.0 (Woodbridge, CT).
7
Bioluminescent Imaging of Tumor Targeting
8
Luciferase Expression in BALB/c Mice
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BALB/c mice bearing ∼50 mm3 TUBO tumors were treated with 108 PFU of Adv/Luc. Expression of luciferase is measured by live animal luminescence imaging using the In Vivo MS FX Pro (Carestream, Woodbridge, CT) after 150 mg/kg i.p. injection of the substrate D-luciferin (Caliper Life Sciences, 119222).
9
Bioluminescence Imaging in Mice
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Mice were depilated and scanned after intraperitoneal delivery of 150 mg/kg of D‐luciferin. Animals were maintained under 1% gas anesthesia during scanning. Images were captured using In Vivo MS FX PRO (Carestream Health, Rochester, NY) and analyzed using Carestream MI SE version 5.0.6.20, 1 exposure of 90‐second duration.
10
DNA Fragmentation Assay for Cancer Cells
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HeLa, SiHa, and C33A cells were plated at a density of 2.5 × 105 cells/ml for 24 h at 37°C under a 5% CO2 atmosphere. The cells were pre-incubated with or without N-acetylcysteine (5 mM) for 2 h. Afterward, the cells were treated with the proanthocyanidin polymer-rich fraction (IC50 and IC90 according to cell line) for 24 h. The cells were washed with PBS and lysed in a solution that contained Tris-HCl (10 mM: pH 8.0), EDTA (1 mM), NaCl (100 mM), SDS (0.5%), and proteinase K (20 mg/ml) at 65°C for 15 min. RNAse (1 mg/ml) was added and incubated at 37°C for 15 min. DNA was extracted by the phenol:chloroform:isoamyl alcohol method (25:24:1; v/v). DNA electrophoresis was performed on 1.0% agarose gel in Tris/boric acid buffer at 90 V for 1 h, and DNA was stained with SYBR safe DNA gel stain (Invitrogen). The molecular weight marker was the 100 bp DNA Ladder. Images were obtained using In vivo MS FX PRO (Carestream Molecular Imaging, Carestream Health).
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