Log In Sign up
The largest database of trusted experimental protocols

Anti cd45 fitc mabs clone hi30

Manufactured by BioLegend
Visit Supplier

Anti-CD45-FITC mAbs (clone HI30) is a monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) that binds to the CD45 antigen expressed on the surface of human leukocytes.

Automatically generated - may contain errors

Lab products found in correlation

Metamorph software, by Molecular Devices (2 mentions) Orca 03g, by Hamamatsu Photonics (2 mentions) Pan ck efluor 615 clone c 11, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-CD45 (228 citations) CD45-FITC (129 citations) Anti-CD45-FITC (54 citations) Clone HI30 (12 citations) CD45 (HI30; (8 citations) CD45 (clone HI30, (7 citations) Anti-CD45 (clone HI30) (3 citations) Anti-CD45 (HI30, (3 citations) CD45-FITC (clone HI30, (2 citations) CD45-FITC (HI30, (2 citations)

2 protocols using anti cd45 fitc mabs clone hi30

1

Immunophenotyping of Circulating Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with anti-CD45-FITC mAbs (clone HI30; BioLegend, San Diego, CA), fixed with formaldehyde (2%), permeabilized with 0.1% Triton X-100, and stained with a mixture of CK 8, (clone C-46), 18 (clone DA/7), 19 (clone A53-B/A2), or pan-CK-eFluor®615 (clone C-11; BioLegend), anti-Vimentin-Cy5, and DAPI. In some cases, cells were stained with anti-EpCAM-Cy5 or FAPα via a secondary IgG mAb. CTC visualization/enumeration was performed using an inverted Olympus IX71 microscope (Center Valley, PA) equipped with a high resolution (1344 × 1024) CCD camera (Hamamatsu ORCA-03G) and a mercury arc lamp. Images were collected, background corrected, normalized, and analyzed using Metamorph software (Molecular Devices Inc.). In ImageJ images were converted to 8-bit type, a gray scale values of the signal were read from the line plots and the phenotypes were classified as no signal (−) (0–30 level), weak (+) (31–100 level), and strong (++) (101–256 level).
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
+ Open protocol
+ Expand
2

CTC Enumeration and Phenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with anti-CD45-FITC mAbs (clone HI30; BioLegend, San Diego, CA), fixed with formaldehyde (2%), permeabilized with 0.1% Triton X-100, and stained with a mixture of CK 8, (clone C-46), 18 (clone DA/7), 19 (clone A53-B/A2), or pan-CK-eFluor®615 (clone C-11; BioLegend), anti-Vimentin-Cy5, and DAPI. In some cases, cells were stained with anti-EpCAM-Cy5 or FAPα via a secondary IgG mAb. CTC visualization/enumeration was performed using an inverted Olympus IX71 microscope (Center Valley, PA) equipped with a high resolution (1344 × 1024) CCD camera (Hamamatsu ORCA-03G) and a mercury arc lamp. Images were collected, background corrected, normalized, and analyzed using Metamorph software (Molecular Devices Inc.). In ImageJ images were converted to 8-bit type, a gray scale values of the signal were read from the line plots and the phenotypes were classified as no signal (−) (0–30 level), weak (+) (31–100 level), and strong (++) (101–256 level).
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!