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Defined hyclone fbs

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Defined Hyclone Fetal Bovine Serum (FBS) is a high-quality, sterile-filtered, and heat-inactivated serum product used as a cell culture supplement. It is derived from the blood of bovine fetuses and provides a source of growth factors, vitamins, and other nutrients to support the in vitro growth and maintenance of a wide range of cell types.

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Lab products found in correlation

Sk mel 28, by American Type Culture Collection (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Human il 4, by Immunotools (2 mentions) Anti cd14 magnetic microbeads, by Miltenyi Biotec (2 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions) Crl 1469, by American Type Culture Collection (1 mentions) Mycoplasma pcr detection kit, by American Type Culture Collection (1 mentions) Eagle s minimum essential medium emem, by American Type Culture Collection (1 mentions) Dulbecco s modified eagle s medium dmem, by American Type Culture Collection (1 mentions) Htb 22, by American Type Culture Collection (1 mentions) Lps eb, by InvivoGen (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) Fungizone, by Merck Group (1 mentions) Human granulocyte macrophage colony stimulating factor, by Immunotools (1 mentions)

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HyClone (528 citations) HyClone FBS (61 citations) Defined FBS (6 citations)

9 protocols using defined hyclone fbs

1

Growth and Maintenance of SK-Mel-28 Cells

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SK-Mel-28 (ATCC, Manassas, VA, USA), a human metastatic melanoma cell line, was grown in high D-glucose DMEM, with 10% (v/v) heat inactivated fetal bovine serum (FBS Defined Hyclone; ThermoFisher Scientific, Waltman, Massachusetts, USA), 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mmol/L glutamine in a humidified atmosphere with 5% CO2 in air. The culture medium was changed every 2 days.
Cecchi M., Paccosi S., Silvano A., Eid A.H, & Parenti A. (2021). Dexamethasone Induces the Expression and Function of Tryptophan-2-3-Dioxygenase in SK-MEL-28 Melanoma Cells. Pharmaceuticals, 14(3), 211.
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2

Cell Culture of Human Cancer and Endothelial Cells

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A human metastatic melanoma cell line, SK-Mel-28 (ATCC, Manassas, VA), a human colon adenocarcinoma cell line, HCT8, and HUVEC cells (ECACC, Salisbury UK) were grown on high D-glucose DMEM, RPMI and on M199, respectively. Media were supplemented with 10% (v/v) heat inactivated fetal bovine serum (FBS Defined Hyclone; Thermo Scientific, Waltman, MA), 100 U/mL penicillin, 100 μg/mL streptomycin and 2 mmol/L glutamine in a humidified atmosphere with 5% CO2 in air. The culture medium utilized, a “complete medium”, was changed every 2 days.
Paccosi S., Cecchi M., Silvano A., Fabbri S, & Parenti A. (2020). Different effects of tryptophan 2,3-dioxygenase inhibition on SK-Mel-28 and HCT-8 cancer cell lines. Journal of Cancer Research and Clinical Oncology, 146(12), 3155-3163.
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3

Cell Culture Protocols for Hematological Malignancies

Cited 2 times
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293T human embryonic kidney cells were cultured in IMDM (Life Technologies Corporation) containing 10% FBS (Hyclone Defined FBS, Thermo Fisher Scientific), 100 U/mL penicillin and 10 μg/mL streptomycin (Life Technologies Corporation). The following human cell lines were cultured in RPMI (Life Technologies Corporation) containing 10% FBS (Atlanta Biologicals, Lawrenceville, GA): IM-9 (EBV-transformed B lymphoblastoid); U266, KMS-11, RPMI 8226, MM.1S (multiple myeloma); Jurkat (acute T cell leukemia); MOLT-4 and CCRF-CEM (acute T-lymphoblastic leukemia); and Z-138 and REC-1 (mantle cell lymphoma) cell lines. KMS-11 cells were a kind gift from Dr. Lawrence Boise (Emory University, Atlanta, GA). All other cell lines were purchased from ATCC (Manassas, VA). TfR1 expression on the surface of these cell lines has been previously shown for IM-9 and U266 [30 (link), 34 (link)], KMS-11 [33 (link)], and Jurkat [30 (link)]. Expression on the surface of the other cell lines is shown in Supplementary Figure 1.
Leoh L.S., Morizono K., Kershaw K.M., Chen I.S., Penichet M.L, & Daniels-Wells T.R. (2014). Gene delivery in malignant B cells using the combination of lentiviruses conjugated to anti-transferrin receptor antibodies and an immunoglobulin promoter. The journal of gene medicine, 16(0), 11-27.
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4

Quantitative RT-PCR Analysis of Pluripotent Stem Cells

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ESCs were maintained on gelatin-coated tissue culture dishes in GMEM (Gibco) supplemented with non-essential amino acids (NEAA, ThermoFisher Scientific), sodium pyruvate, GlutaMAX™, 50 μM β-mercaptoethanol (all from ThermoFisher Scientific), 10% fetal bovine serum (FBS, HyClone™ Defined FBS) and 10 ng/ml LIF. RNA isolation and quantitative RT-PCR (qRT-PCR) from ESCs were performed as previously described [44 (link)]. Primer details can be found in Table 3. Relative initial mRNA concentrations were estimated by curve fitting, and normalized to Ppia housekeeping gene mRNA levels in each sample.
Morgani S.M., Saiz N., Garg V., Raina D., Simon C.S., Kang M., Arias A.M., Nichols J.N., Schröter C, & Hadjantonakis A.K. (2018). A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Developmental biology, 441(1), 104-126.
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5

Established Cell Lines for Cancer Research

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An established breast adenocarcinoma cell line, MCF-7 (ATCC, HTB-22™), and an established pancreatic adenocarcinoma cells line, PANC-1 (ATCC, CRL-1469™), were used throughout this study. All cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA) as a frozen vial and maintained as described in the technical bulletins in a 37°C cell culture incubator with 5% CO2. Cells were maintained in the recommended basal medium supplemented with 10% defined fetal bovine serum (FBS) and 1% v/v 100X antibiotics/antimycotics (Ab/Am). Cells were tested for mycoplasma contamination using a commercial mycoplasma PCR detection kit (ATCC) and confirmed to be free of mycoplasma contamination following all experimental procedures. Eagle's Minimum Essential Medium (EMEM) and Dulbecco's Modified Eagle's Medium (DMEM) were obtained from ATCC. Hyclone defined FBS and Gibco 100X Ab/Am, a cocktail of 10,000 U/mL penicillin, 10,000 μg/mL streptomycin, and 25 μg/mL amphotericin B, were obtained from Thermo Fisher Scientific (Waltham, MA).
Gillespie J.W., Gross A.L., Puzyrev A.T., Bedi D, & Petrenko V.A. (2015). Combinatorial synthesis and screening of cancer cell-specific nanomedicines targeted via phage fusion proteins. Frontiers in Microbiology, 6, 628.
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6

Monocyte-Derived Dendritic Cell Activation

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Six day old monocyte-derived dendritic cells were plated in 96-well plates at 2 × 105 cells in 200 µL RPMI-1640 media supplemented with 10% defined Hyclone FBS (Thermo Scientific) and stimulated with a 1:2 dilution of control TCM or pyrazinib (P3) treated TCM from OAC tumour explants or 0.1% DMSO or 10 µM pyrazinib (P3) for 4–5 h before exposure to 10 µg/mL of ultra-pure TLR4 agonist Escherichia coli lipopolysaccharide (LPS-EB; Invivogen) overnight, incubating at 37 °C, 5% CO2. Supernatants were harvested and snap frozen, and cells were assessed for expression of surface markers.
Buckley A.M., Dunne M.R., Morrissey M.E., Kennedy S.A., Nolan A., Davern M., Foley E.K., Clarke N., Lysaght J., Ravi N., O’Toole D., MacCarthy F., Reynolds J.V., Kennedy B.N, & O’Sullivan J. (2020). Real-time metabolic profiling of oesophageal tumours reveals an altered metabolic phenotype to different oxygen tensions and to treatment with Pyrazinib. Scientific Reports, 10, 12105.
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7

Dendritic Cell Activation by LPS

Cited 2 times
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Freshly generated DCs were plated in 96-well plates at 2 × 105 cells in 200 μL RPMI-1640 media supplemented with 10% defined Hyclone FBS (Thermo Fisher Scientific) and stimulated with a 1:2 dilution of conditioned media, or matched background media controls, for 4–5 h before exposure to 10 μg ml-1 of ultrapure TLR4 agonist Escherichia coli lipopolysaccharide (LPS-EB; Invivogen) overnight. Supernatants were harvested and frozen for ELISA analysis, and cells were assessed for expression of surface markers by flow cytometry as we described previously (Supplementary Fig. 1 A) [18 (link), 20 (link), 39 (link)–41 (link)].
Morrissey M.E., Byrne R., Nulty C., McCabe N.H., Lynam-Lennon N., Butler C.T., Kennedy S., O’Toole D., Larkin J., McCormick P., Mehigan B., Cathcart M.C., Lysaght J., Reynolds J.V., Ryan E.J., Dunne M.R, & O’Sullivan J. (2020). The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer, 20, 566.
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8

Human Monocyte-Derived Dendritic Cell Generation

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Human monocyte-derived immature dendritic cells were generated from peripheral blood mononuclear cells (PBMCs) obtained from buffy coat preparations (National Blood Centre, St. James’s Hospital, Dublin) by density gradient centrifugation (Lymphoprep) as described43 (link),48 (link). Briefly, monocytes were isolated by positive selection using anti-CD14 magnetic microbeads, as described by the manufacturer (Miltenyi Biotec) and seeded at a density of 1 × 106 cells/mL in 6-well plates in 3 mL of Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco) containing 10% defined HyClone FBS (Thermo Scientific), 1% penicillin/streptomycin (Lonza, Basel, Switzerland), 1% fungizone (Sigma-Aldrich), human granulocyte macrophage colony-stimulating factor (GM-CSF, 50 ng/mL; Immunotools, Germany), and human IL-4 (70 ng/mL; Immunotools) in a humidified atmosphere with 5% CO2 at 37 °C. Cells were fed at day 3 by replacing half the medium with fresh cytokine-supplemented RPMI. At day 6, cells exhibited a CD11c+ immature dendritic cell phenotype.
Buckley A.M., Dunne M.R., Morrissey M.E., Kennedy S.A., Nolan A., Davern M., Foley E.K., Clarke N., Lysaght J., Ravi N., O’Toole D., MacCarthy F., Reynolds J.V., Kennedy B.N, & O’Sullivan J. (2020). Real-time metabolic profiling of oesophageal tumours reveals an altered metabolic phenotype to different oxygen tensions and to treatment with Pyrazinib. Scientific Reports, 10, 12105.
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9

Generation of Immature Monocyte-Derived DCs

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Human monocyte-derived immature DCs were generated from peripheral blood mononuclear cells (PBMCs) obtained from buffy coat preparations (National Blood Centre, St. James’s Hospital, Dublin) by density gradient centrifugation (Lymphoprep) as described [29 (link), 30 (link)]. Briefly, monocytes were isolated by positive selection using anti-CD14 magnetic microbeads as described by the manufacturer (Miltenyi Biotec) and seeded at a density of 1 × 106 cells/ml in 6-well plates in 3 ml of RPMI-1640 medium containing 10% defined HyClone FBS (Thermo Scientific), 1% penicillin/streptomycin, 1% fungizone, human granulocyte macrophage colony-stimulating factor (50 ng/ml; Immunotools), and human IL-4 (70 ng/ml; Immunotools) in a humidified atmosphere with 5% CO2 at 37 °C. Cells were fed at day 3 by replacing half the medium made up with fresh cytokines. At day 6, CD11c+ cells exhibited an immature DC phenotype, as confirmed by ability to upregulate maturation markers following LPS stimulation.
Kennedy S.A., Morrissey M.E., Dunne M.R., O’Connell F., Butler C.T., Cathcart M.C., Buckley A.M., Mehigan B.J., Larkin J.O., McCormick P., Kennedy B.N, & O’Sullivan J. (2020). Combining 1,4-dihydroxy quininib with Bevacizumab/FOLFOX alters angiogenic and inflammatory secretions in ex vivo colorectal tumors. BMC Cancer, 20, 952.
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