Lv atg7

The NP cells reaching 40‐60% confluence were transfected using lentivirus (LV‐siHuR was from Cell‐land, China, TACCAGTTTCAATGGTCATAA; LV‐HuR was from OBiO, China, NM_001108848; and LV‐Atg7 was from GeneChem, China, NM_001012097) at a multiplicity of infection (MOI) of 50.

Sodium taurocholate (Na-TC) and sodium pentobarbital were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lentiviral vectors encoding ATG7 (Lv-ATG7) or CAMKII (Lv-CAMKII) and lentivirus with scrambled sh-ATG7 (Lv-sh-ATG7) or sh-CAMKII (Lv-sh-CAMKII) were purchased from GeneChem (Shanghai, China). MiR-30b-5p mimic and mimic negative control were purchased from RiboBio (Guangzhou, China). The primary antibodies used for Western blot were microtubule-associated protein 1 light chain 3 (LC3), p62, ATG7 and high mobility group protein B1 (HMGB1) purchased from Cell Signaling Technology (Danvers, MA, USA), tumor necrosis factor α (TNF-α), β-actin and interleukin-1β (IL-1β) purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), CAMKII purchased from Abcam (Shanghai, China), and lysosome-associated membrane protein-2 (LAMP-2) purchased from Thermo Fisher Scientific (Rockford, IL, USA).