Rabbit anti cd63 ls c204227

Manufactured by LifeSpan BioSciences

Rabbit anti-CD63 (LS-C204227) is a laboratory reagent used for the detection and analysis of CD63 protein. CD63 is a member of the tetraspanin family of proteins and is commonly used as a marker for exosomes and late endosomes/lysosomes. This product is a polyclonal antibody raised in rabbits and can be used in various immunoassay techniques to identify and quantify CD63 in biological samples.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Pkh67 membrane dye, by Merck Group (2 mentions) Amicon ultra centrifugal filter unit, by Merck Group (2 mentions) Rabbit anti rab7 d95f2, by Cell Signaling Technology (2 mentions) Rabbit anti lamp1 1d4b, by Abcam (2 mentions) Rabbit anti eea1 c45b10, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit anti cd63 ls c204227

Labeling and Imaging of Extracellular Vesicles

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Following sEV preparation by PEG precipitation and ultracentrifugation from the supernatant of hCMEC/D3, as described, sEV preparations were labeled with PKH67 membrane dye (Sigma-Aldrich). Briefly, sEV preparations were stained with 2 µM PKH67 diluted in diluent C for 5 min at room temperature. Excess dye was removed by washing twice with PBS using Amicon-Ultra Centrifugal Filter Units (Merck Millipore). Labeled sEVs were incubated with hCMEC/D3 for 24 h. Cells were fixed with PFA, labeled with rabbit anti-Rab7 (D95F2; Cell Signaling Technology), rabbit anti-CD63 (LS-C204227; Lifespan Biosciences) or rabbit anti-LAMP1 (1D4B; Abcam) antibody, detected by appropriate secondary antibody (as above) and counterstained with Hoechst 33342 (Thermo Fisher Scientific).

Mohamud Yusuf A., Hagemann N., Zhang X., Zafar M., Hussner T., Bromkamp C., Martiny C., Tertel T., Börger V., Schumacher F., Solari F.A., Hasenberg M., Kleinschnitz C., Doeppner T.R., Kleuser B., Sickmann A., Gunzer M., Giebel B., Kolesnick R., Gulbins E, & Hermann D.M. (2022). Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles. Basic Research in Cardiology, 117(1), 43.

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Tracking sEV Uptake in hCMEC/D3 Cells

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Following sEV preparation by PEG precipitation and ultracentrifugation from the supernatant of hCMEC/D3, as described, sEV preparations were labeled with PKH67 membrane dye (Sigma-Aldrich). Briefly, sEV preparations were stained with 2 µM PKH67 diluted in diluent C for 5 min at room temperature. Excess dye was removed by washing twice with PBS using Amicon-Ultra Centrifugal Filter Units (Merck Millipore). Labeled sEVs were incubated with hCMEC/D3 for 24 hours. Cells were fixed with PFA, labeled with rabbit anti-EEA1 (C45B10; Cell Signaling Technology), rabbit anti-Rab7 (D95F2; Cell Signaling Technology), rabbit anti-CD63 (LS-C204227; Lifespan Biosciences) or rabbit anti-Lamp1 (1D4B; Abcam) antibody, detected by appropriate secondary antibody (as above) and counterstained with Hoechst 33342 (Thermo Fisher Scientific).

Yusuf A.M., Hagemann N., Zhang X., Zafar M.U., Hussner T., Bromkamp C., Martiny C., Tertel T., Börger V., Schumacher F., Solari F., Hasenberg M., Kleinschnitz C., Doeppner T., Kleuser B., Sickmann A., Gunzer M., Giebel B., Kolesnick R., Gulbins E., & Hermann D. (2021). Acid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesicles.

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