The antimicrobial susceptibility test was done with the disk diffusion method (Bauer et al, 1966) using Mueller–Hinton agar (Difco). Initially, an emulsion of sample in saline solution was prepared by adjustment to the 0.5 McFarland turbidity standards. The susceptibility of the E. coli strains was tested in relation to several antibiotics, including: chloramphenicol (CAF) (30 μg), ciprofloxacin (5 μg), gentamycin (10 μg), trimethoprim (1.25 μg-sulfamethoxazole 23.75 μg), erythromycin (15 μg), streptomycin (10 μg) and tetracycline (30 μg) (Mast Group Ltd., Merseyside, UK). Using sterile tweezers, commercially available antibiotic disks were placed individually on the surface of Mueller-Hinton agar. After 24 h of incubation at 35°C, the strains were scored as “susceptible”, “intermediate”, or “resistant” to each antibiotic based on the measurement of the inhibition zone, as recommended by clinical laboratory standard institute (CLSI).26
Streptomycin
Manufactured by Mast Group
Sourced in United Kingdom
Streptomycin is a laboratory-grade antibiotic compound used in various research and analytical applications. It functions as a bacteriostatic agent, inhibiting the growth and reproduction of certain bacterial species.
Automatically generated - may contain errors
Lab products found in correlation
Tetracycline, by Mast Group (2 mentions)
Chloramphenicol, by Mast Group (2 mentions)
Erythromycin, by Mast Group (2 mentions)
Gentamycin, by Mast Group (1 mentions)
Trimethoprim, by Mast Group (1 mentions)
Ciprofloxacin, by Mast Group (1 mentions)
Ampicillin, by Mast Group (1 mentions)
Penicillin, by Mast Group (1 mentions)
Vancomycin, by Mast Group (1 mentions)
Kanamycin, by Mast Group (1 mentions)
Clindamycin, by Mast Group (1 mentions)
Gentamicin, by Mast Group (1 mentions)
2 protocols using streptomycin
1
Antimicrobial Susceptibility Profiling of E. coli
2
Antibiotic Resistance Profiling of Bacterial Isolates
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To assess the antibiotic resistance of the isolates, the disc diffusion method (DDM) and Micro dilution methods were applied62 (link). The sensitivities of the bacteria to antibiotics were interpreted as sensitive (S), intermediate (I), and resistant (R) based on the Clinical & Laboratory Standards Institute (CLSI) guidelines63 . The Antibiotic discs used were chloramphenicol (30 µg/disc), gentamicin (10 µg/disc), erythromycin (15 µg/disc), clindamycin (2 µg/disc), kanamycin (30 µg/disc), vancomycin (30 µg/disc), penicillin (10 IU/disc), streptomycin (10 µg/disc), tetracycline (30 µg/disc) and ampicillin (10 µg/disc) that were purchased from MAST Group (Merseyside, UK). To evaluate the minimum inhibitory concentrations (MICs) of these ten antibiotics, different amounts of each antibiotic were diluted in broth medium to prepare the following concentrations: 0.5 to 256 μg/mL (chloramphenicol), 0.125 to 64 μg/mL (clindamycin, erythromycin, and ampicillin) and 0.25 to 128 μg/mL (vancomycin, tetracycline). The strains treated with these antibiotics were incubated at 35 °C for 24 h. The minimum bactericidal concentrations (MBCs) were determined as described by CLSI63 .
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