Uplc optimized fluorescence detector

AF identification was carried out according to the technique proposed by Jardon-Xicontencatl et al. [12 (link)] using a Waters ACQUITY Ultra Performance Liquid Chromatography (UPLC) H-Class System equipped with a quaternary solvent manager and an ACQUITY UPLC BEH C18 phase reverse column (2.1 × 100 mm, 1.7 μm). Standards, as well as samples collected from the IAC (1 μL) were injected and eluted with a single ternary mixture of 64:18:18 water/methanol/acetonitrile (all HPLC grade) at a flow rate of 400 μL/min. AF were fluorometrically detected and identified using an UPLC-optimized fluorescence detector (Waters, Milford, MA, USA). The excitation and emission wavelengths were 365 and 429 nm, respectively. AF were identified by their retention time (Rt) and compared with those for a pure AF standard solution under identical conditions. The estimated detection limits are 0.58 and 2.01 ng/L for AFB₂ and AFB₁, respectively.

The aflatoxin identification was carried out according to the technique proposed by Jardon-Xicontencatl et al. [20 (link)], using a Waters ACQUITY UPLC H-Class System equipped with a quaternary solvent manager and an ACQUITY UPLC BEH C18 phase reverse column (2.1 × 100 mm, 1.7 µm). Standards, as well as samples collected from the IACs (1 µL), were injected and eluted with a single ternary mixture of 64:18:18 water/methanol/acetonitrile (all HPLC grade) at a flow rate of 400 µL/min. Aflatoxins were fluorometrically detected and identified using a UPLC-optimized fluorescence detector (Waters, Milford, MA, USA). The excitation and emission wavelengths were 365 and 429 nm, respectively. Aflatoxins were identified by their Rt and compared with those of a pure aflatoxin standard solution. The estimated detection limits are 0.58 and 2.01 ng/kg for AFB₂ and AFB₁, respectively.