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R2200 ultrahigh resolution spectral domain oct system

Manufactured by Bioptigen

The R2200 ultrahigh resolution spectral domain OCT system is a laboratory equipment designed for high-resolution optical coherence tomography imaging. It provides a non-invasive, cross-sectional imaging capability with micrometer-scale resolution. The core function of the R2200 is to capture and display detailed images of biological samples or structures.

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2 protocols using r2200 ultrahigh resolution spectral domain oct system

1

Quantifying Mouse ONL Thickness via OCT

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Live fundus images were obtained with a Micron III Retinal Imaging System (Phoenix Research Laboratories) and an R2200 ultrahigh resolution spectral domain OCT system (Bioptigen). Image acquisition and processing were performed as described previously.58 (link) ONL thickness in one eye of each mouse was determined from OCT volume datasets imported into Fiji at four positions, each ∼250 μm from the optic nerve head in the superior, nasal, temporal and temporal fundus. Thickness was determined from 50-pixel wide lines drawn vertically across the ONL at each position. To identify the ONL boundaries reproducibly, the Plot Profile tool was used to plot image intensity by distance, and the length (in pixels) of a horizontal line between the midpoints of the intensity peaks on either side of the ONL was determined. Values from each eye were averaged and converted to ONL thickness by an axial factor of 1.53 μm/pixel determined from the OCT acquisition program.
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2

Quantifying Mouse ONL Thickness via OCT

Check if the same lab product or an alternative is used in the 5 most similar protocols
Live fundus images were obtained with a Micron III Retinal Imaging System (Phoenix Research Laboratories) and an R2200 ultrahigh resolution spectral domain OCT system (Bioptigen). Image acquisition and processing were performed as described previously.58 (link) ONL thickness in one eye of each mouse was determined from OCT volume datasets imported into Fiji at four positions, each ∼250 μm from the optic nerve head in the superior, nasal, temporal and temporal fundus. Thickness was determined from 50-pixel wide lines drawn vertically across the ONL at each position. To identify the ONL boundaries reproducibly, the Plot Profile tool was used to plot image intensity by distance, and the length (in pixels) of a horizontal line between the midpoints of the intensity peaks on either side of the ONL was determined. Values from each eye were averaged and converted to ONL thickness by an axial factor of 1.53 μm/pixel determined from the OCT acquisition program.
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