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Anti cd68 mouse igg3

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Anti-CD68 (mouse IgG3, ) is a laboratory reagent used for the detection and identification of CD68 protein. CD68 is a glycoprotein expressed primarily by macrophages and monocytes. This antibody can be used in various immunochemical techniques, such as immunohistochemistry and flow cytometry, to study the distribution and expression of CD68 in biological samples.

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Lab products found in correlation

Bx60 microscope, by Olympus (2 mentions) Dp73 camera, by Olympus (2 mentions) Mach4 universal hrp polymer kit, by Biocare Medical (2 mentions) Ferangi blue chromogen kit, by Biocare Medical (2 mentions) Cell sens standard ink imaging software, by Olympus (2 mentions) Anti iba 1 rabbit polyclonal, by Fujifilm (2 mentions)

Close spelling variants of this product

Anti-CD68 (66 citations) Mouse anti-CD68 (11 citations) Anti-TM (1 citations)

2 protocols using anti cd68 mouse igg3

1

Immunohistochemical Analysis of Microglia Activation

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Histological diagnosis was revised and formalin-fixed paraffin-embedded representative sections for each patient were selected based on adequate tissue preservation as assayed by hematoxylin and eosin (H&E) staining and subjected to IHC. Briefly, 20μm thick paraffin embedded representative tissue sections were de-waxed, rehydrated and endogenous peroxidase activity blocked with 0,3% H2O2 in methanol for 20min. Antigen retrieval was performed by using a microwave-oven in 1mM Citrate buffer (pH 6.0). Sections were then washed in TBS (pH 7.4) and incubated for one hour or overnight in anti-Iba1 (rabbit polyclonal, 1:300; Wako), anti-CD68 (mouse IgG3, 1:100; Dako) or, anti-HLA-DR (1:250, Biomeda) in TBS 1% BSA. Signal was revealed using the DAKO Envision+System-HRP Labelled Polymer Anti-Rabbit or Anti-Mouse or the NovoLinkTM Polymer Detection System (NovocastraTM), followed by Diaminobenzydine (DAB) as chromogen and Hematoxylin as counterstain. For detection of microglia around the plaques, we combined silver staining with Iba1 IHC by using the MACH4 Universal HRP-Polymer kit (Biocare) and signal was revealed by Ferangi Blue Chromogen Kit (Biocare). Images were acquired with an Olympus Bx60 microscope and Cell Sens Standard Ink imaging software (Olympus Corporation) mounted on a DP73 Olympus camera.
Zhou Y., Song W.M., Andhey P.S., Swain A., Levy T., Miller K.R., Poliani P.L., Cominelli M., Grover S., Gilfillan S., Cella M., Ulland T.K., Zaitsev K., Miyashita A., Ikeuchi T., Sainouchi M., Kakita A., Bennett D.A., Schneider J.A., Nichols M.R., Beausoleil S.A., Ulrich J., Holtzman D.M., Artyomov M.N, & Colonna M. (2020). Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and - independent cellular responses in Alzheimer’s disease. Nature medicine, 26(1), 131-142.
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2

Immunohistochemical Analysis of Microglia Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Histological diagnosis was revised and formalin-fixed paraffin-embedded representative sections for each patient were selected based on adequate tissue preservation as assayed by hematoxylin and eosin (H&E) staining and subjected to IHC. Briefly, 20μm thick paraffin embedded representative tissue sections were de-waxed, rehydrated and endogenous peroxidase activity blocked with 0,3% H2O2 in methanol for 20min. Antigen retrieval was performed by using a microwave-oven in 1mM Citrate buffer (pH 6.0). Sections were then washed in TBS (pH 7.4) and incubated for one hour or overnight in anti-Iba1 (rabbit polyclonal, 1:300; Wako), anti-CD68 (mouse IgG3, 1:100; Dako) or, anti-HLA-DR (1:250, Biomeda) in TBS 1% BSA. Signal was revealed using the DAKO Envision+System-HRP Labelled Polymer Anti-Rabbit or Anti-Mouse or the NovoLinkTM Polymer Detection System (NovocastraTM), followed by Diaminobenzydine (DAB) as chromogen and Hematoxylin as counterstain. For detection of microglia around the plaques, we combined silver staining with Iba1 IHC by using the MACH4 Universal HRP-Polymer kit (Biocare) and signal was revealed by Ferangi Blue Chromogen Kit (Biocare). Images were acquired with an Olympus Bx60 microscope and Cell Sens Standard Ink imaging software (Olympus Corporation) mounted on a DP73 Olympus camera.
Zhou Y., Song W.M., Andhey P.S., Swain A., Levy T., Miller K.R., Poliani P.L., Cominelli M., Grover S., Gilfillan S., Cella M., Ulland T.K., Zaitsev K., Miyashita A., Ikeuchi T., Sainouchi M., Kakita A., Bennett D.A., Schneider J.A., Nichols M.R., Beausoleil S.A., Ulrich J., Holtzman D.M., Artyomov M.N, & Colonna M. (2020). Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and - independent cellular responses in Alzheimer’s disease. Nature medicine, 26(1), 131-142.
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