P. falciparum NF54 sporozoites dissected from salivary glands were stained with 1:2000 SYBR Green and four dilutions of VRC 314 U.S. plasma (1:20, 1:100, 1:500, and 1:2500) for 30 minutes at 4˚C, washed, and then incubated with 2.5 μg/mL goat anti-human IgA-Alexa Fluor 647 (Jackson Immunoresearch 109-606-011) or goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170) for 30 minutes at 4˚C. The binding data with the sporozoites were acquired with the iQue Screener Plus high-throughput flow cytometer and analyzed with FlowJo software (Tree Star).
Goat anti human iga alexa fluor 647
Manufactured by Jackson ImmunoResearch
Goat anti-human IgA-Alexa Fluor 647 is a secondary antibody conjugate. It is designed to detect and quantify human immunoglobulin A (IgA) in various immunoassays and research applications. The antibody is produced in goats and is conjugated to the Alexa Fluor 647 fluorescent dye, enabling detection and visualization of target IgA molecules.
Automatically generated - may contain errors
2 protocols using goat anti human iga alexa fluor 647
1
Sporozoite Immunofluorescence Binding Assay
2
Sporozoite Immunofluorescence Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
P. falciparum NF54 sporozoites dissected from salivary glands were stained with 1:2000 SYBR Green and four dilutions of VRC 314 U.S. plasma (1:20, 1:100, 1:500, and 1:2500) for 30 minutes at 4˚C, washed, and then incubated with 2.5 μg/mL goat anti-human IgA-Alexa Fluor 647 (Jackson Immunoresearch 109-606-011) or goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170) for 30 minutes at 4˚C. The binding data with the sporozoites were acquired with the iQue Screener Plus high-throughput flow cytometer and analyzed with FlowJo software (Tree Star).
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P. falciparum NF54 sporozoites dissected from salivary glands were stained with 1:2000 SYBR Green and four dilutions of VRC 314 U.S. plasma (1:20, 1:100, 1:500, and 1:2500) for 30 minutes at 4˚C, washed, and then incubated with 2.5 μg/mL goat anti-human IgA-Alexa Fluor 647 (Jackson Immunoresearch 109-606-011) or goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170) for 30 minutes at 4˚C. The binding data with the sporozoites were acquired with the iQue Screener Plus high-throughput flow cytometer and analyzed with FlowJo software (Tree Star).
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