Rab10

Manufactured by Merck Group

Rab10 is a protein that plays a key role in cellular transport and trafficking processes. It is a member of the Rab family of small GTPases, which are involved in the regulation of vesicle formation, movement, and fusion within the cell. Rab10 specifically functions in the transport of cargo between the endoplasmic reticulum and the Golgi apparatus, as well as in the regulation of insulin-stimulated glucose uptake in adipocytes.

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Lab products found in correlation

Lenti x concentrator, by Takara Bio (1 mentions) Lrrk2 shrna, by Merck Group (1 mentions) Pspax2, by Addgene (1 mentions) X tremegene hp dna transfection reagent, by Roche (1 mentions) Tgn46, by Merck Group (1 mentions) Gapdh, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using rab10

Lentiviral Vector Construction for Rab10 and LRRK2

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Rab8, Rab10, and LRRK2 shRNA constructs were obtained from Sigma. Rab10 WT and T72E were cloned into the pER4 lentiviral expression vector using standard cloning techniques. Lentiviral vectors along with helper plasmids, pLP3 (Invitrogen) and psPAX2 (Addgene, #12260), were transfected into HEK-FT cells using X-treme Gene HP DNA Transfection Reagent (Roche). The resulting virus particles were purified and concentrated using LentiX-concentrator (Clontech). Virus titers were determined using the RETROtek p24 Antigen ELISA kit (ZeptoMetric Corporation). Concentrated virus was stored in single use aliquots at −80 °C.

Ysselstein D., Nguyen M., Young T.J., Severino A., Schwake M., Merchant K, & Krainc D. (2019). LRRK2 kinase activity regulates lysosomal glucocerebrosidase in neurons derived from Parkinson’s disease patients. Nature Communications, 10, 5570.

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SDS-PAGE and Western Blot Analysis

Cited 1 time

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Cell lysates or gradient centrifugation fractions were analysed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot as previously described (Troese et al., 2011 (link)). Commercially available primary antibodies targeted β-actin (Santa Cruz Biotechnology, Santa Cruz, CA), GAPDH (Sigma-Aldrich), GFP (Invitrogen), HaloTag (Promega), TGN46 (Sigma-Aldrich) and Rab10 (Sigma-Aldrich). Rabbit antisera against A. phagocytophilum proteins P44 (IJdo et al., 1999 (link)) and APH0032 (Huang et al., 2010b (link)) were described previously. Rabbit antibody targeting a surface-exposed epitope of Asp55 (Ge and Rikihisa, 2007 (link)) was a kind gift from Yasuko Rikihisa (Ohio State University, Columbus, OH).

Truchan H.K., VieBrock L., Cockburn C.L., Ojogun N., Griffin B.P., Wijesinghe D.S., Chalfant C.E, & Carlyon J.A. (2015). Anaplasma phagocytophilum Rab10-dependent parasitism of the trans-Golgi network is critical for completion of the infection cycle. Cellular microbiology, 18(2), 260-281.

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