Em vcm

Manufactured by Leica camera

The EM VCM is a vacuum control module designed for use with Leica's electron microscopy equipment. It provides precise and reliable control of the vacuum environment within the microscope chamber, which is essential for achieving high-quality imaging and analytical results. The EM VCM is a core component of Leica's comprehensive electron microscopy solutions, engineered to meet the demanding requirements of advanced materials research and characterization.

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Lab products found in correlation

Em vct500, by Leica camera (6 mentions) Em ace900, by Leica camera (6 mentions) Em ice, by Leica camera (5 mentions) Geminisem, by Zeiss (4 mentions) Hrsem gemini 300 sem, by Zeiss (1 mentions)

6 protocols using em vcm

Cryo-Preparation and Imaging of 3D-Bioprinted CPs

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Samples of a cellular, 3D-bioprinted CPs, were fabricated as described above. Dissected samples were placed and sandwiched between two aluminum disks (3 mm in diameter, each 25-μm thick) and cryo-immobilized in a high-pressure freezing device (EM ICE; Leica).
The frozen samples were then mounted on a holder under liquid nitrogen in a specialized loading station (EM VCM; Leica) and transferred under cryogenic conditions (EM VCT500; Leica) to a sample preparation freeze fracture device (EM ACE900; Leica). In that device, the samples were fractured by the rapid stroke of a cryogenically cooled knife, exposing the inner part of the sandwiched disks. After fracturing, the samples were etched at -100°C for 10 min to sublime ice from the sample surface, and were coated with 3 nm carbon.
Samples were imaged in a Gemini SEM (Zeiss) by a secondary electron in-lens detector, while maintaining an operating temperature of −120°C (Figure S3 in Supplementary File). The measurements were done at the Ilse Katz Institute for Nanoscale Science and Technology at Ben-Gurion University of the Negev, Beer Sheva, Israel.

Bar A., Kryukov O., Etzion S, & Cohen S. (2023). Engineered extracellular vesicle-mediated delivery of miR-199a-3p increases the viability of 3D-printed cardiac patches. International Journal of Bioprinting, 9(2), 670.

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Cryo-Fracture and Electron Microscopy

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The samples were placed and sandwiched between two aluminum discs (3 mm in diameter, 25 μm in thick each) and cryo-immobilized in a high-pressure freezing device (EM ICE, Leica).
The frozen samples were then mounted on a holder under liquid nitrogen in a specialized loading station (EM VCM, Leica) and transferred under cryogenic conditions (EM VCT500, Leica) to a sample preparation freeze fracture device (EM ACE900, Leica). In that device, the samples were fractured by a rapid stroke of a cryogenically cooled knife, exposing the inner part of the sandwiched discs. After fractured, the samples were etched at −100 °C for 10 min to sublime ice from the sample surface and coated with 3 nm carbon.
Samples were imaged in a Gemini SEM (Zeiss) by a secondary electron in-lens detector while maintaining an operating temperature of −120 °C. The measurements were done at the Ilse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev, Beer Sheva, Israel.

Bar A., Kryukov O, & Cohen S. (2022). Three-Dimensional Bio-Printed Cardiac Patch for Sustained Delivery of Extracellular Vesicles from the Interface. Gels, 8(12), 769.

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Cryo-SEM Imaging of Scallop Eyes

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Eyes from fixed scallops were sandwiched between two aluminum disks and cryo-immobilized in a high-pressure freezing (HPF) device (EM ICE, Leica). The frozen samples were then mounted on a holder under liquid nitrogen in a specialized loading station (EM VCM, Leica) and transferred under cryogenic conditions (EM VCT500, Leica) to a sample preparation freeze fracture device (EM ACE900, Leica). These samples are held under vacuum at a temperature of −120 °C. Samples are fractured by hitting the top disc carrier with a tungsten knife at a speed of 160 mm/s, this exposed a clean fracture plane which can be imaged. Then the samples were etched for 5 min at −110 °C to uncover additional structural details of the sample by controlled evaporation of water. Lastly, the samples were coated with 3 nm of PtC. Samples were imaged in an HRSEM Gemini 300 SEM (Zeiss) by secondary electron in-lens detector while maintaining an operating temperature of −120 °C. During this study, a total of 28 scallop eyes were examined by cryo-SEM.

Wagner A., Upcher A., Maria R., Magnesen T., Zelinger E., Raposo G, & Palmer B.A. (2023). Macromolecular sheets direct the morphology and orientation of plate-like biogenic guanine crystals. Nature Communications, 14, 589.

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Cryogenic Imaging of BSA-PEGDA Hydrogels

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BSA‐PEGDA hydrogels (0–200, 0.5–200, 2–200, and 2–100 mm) were washed three times with DDH₂O at RT. The hydrogel samples were inserted between two aluminum discs (3 mm in diameter, 25 µm thick each) and then cryo‐immobilized in a high‐pressure freezing device (EM ICE, Leica). The samples were mounted on a holder under liquid nitrogen in a loading station (EM VCM, Leica) and transferred under cryogenic conditions (EM VCT500, Leica) to a sample preparation freeze fracture device (EM ACE900, Leica). The samples were fractured by a rapid stroke of a cryogenically cooled knife to expose the inner part between the discs. Afterward, the samples were etched (−100 °C, for 10 min) to sublimate ice from the sample surface and then coated with 3 nm carbon. A secondary electron in‐lens detector was used to image the samples (Gemini SEM (Zeiss)‐operating temperature of −120 °C). The measurements were done at the Ilse Katz Institute for Nanoscale Science and Technology at the Ben‐Gurion University of the Negev, Beer Sheva, Israel.

Kaeek M, & Khoury L.R. (2023). Toward Tunable Protein‐Driven Hydrogel Lens. Advanced Science, 10(36), 2306862.

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Cryo-Imaging of Cleaner Shrimp Maxillipeds

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White maxillipeds of the cleaner shrimp (not chemically fixed) were cut perpendicular to the length of the limb into ~1-mm sections. The sections were sandwiched between two aluminium discs and cryo-immobilized in a high-pressure freezing device (EM ICE, Leica). The frozen samples were then mounted on a holder under liquid nitrogen in a specialized loading station (EM VCM, Leica) and transferred under cryogenic conditions (EM VCT500, Leica) to a sample preparation freeze-fracture device (EM ACE900, Leica). The samples were freeze-fractured at −120 °C, etched at −110 °C for 3 min, and coated with 3 nm of Pt/C. The samples were imaged in an HRSEM Gemini 300 scanning electron microscope (Zeiss) by a secondary electron in-lens detector while maintaining an operating temperature of −120 °C.

Lemcoff T., Alus L., Haataja J.S., Wagner A., Zhang G., Pavan M.J., Yallapragada V.J., Vignolini S., Oron D., Schertel L, & Palmer B.A. (2023). Brilliant whiteness in shrimp from ultra-thin layers of birefringent nanospheres. Nature Photonics, 17(6), 485-493.

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Cryogenic Ultrastructural Analysis of Shrimp Eyes

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Whole larval shrimp eyes (fixed or live) were sandwiched between two aluminum discs (3 mm in diameter, 0.1 mm cavity), in a 15% dextran in 1X PBS solution, and cryoimmobilized in a high-pressure freezing device (ICE, Leica). The frozen samples were mounted on a holder under liquid nitrogen in a specialized loading station (EM VCM, Leica) and transferred under cryogenic conditions by a vacuum cryo-transfer device (EM VCT500, Leica) to a sample preparation freeze-fracture device (EM ACE900, Leica). The samples were fractured at a speed of 160 mm/sec, etched for 3 minutes at -110°C, and coated with 3 nm PtC. Then, samples were imaged in a Gemini SEM (Zeiss) by a secondary electron in-lens detector while maintaining an operating temperature of -120°C. Particle diameters and their 2D filling fractions were determined from cryo-SEM images for all the shrimp and prawn specimens.

Shavit K., Wagner A., Schertel L., Farstey V., Akkaynak D., Zhang G., Upcher A., Sagi A., Yallapragada V.J., Haataja J, & Palmer B.A. (2023). A tunable reflector enabling crustaceans to see but not be seen. Science (New York, N.Y.), 379(6633).

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