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Enhanced chemiluminiscent ecl solution

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Enhanced chemiluminescent (ECL) solution is a laboratory reagent used in Western blotting techniques for the detection and quantification of proteins. It generates a luminescent signal in the presence of the target protein, which can be captured and analyzed using specialized imaging equipment.

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Lab products found in correlation

Nupage bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Anti phospho met tyr1234 1235, by Cell Signaling Technology (2 mentions) Anti phospho erk, by Cell Signaling Technology (2 mentions) Anti met, by Cell Signaling Technology (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Anti phospho plcγ1 tyr783, by Cell Signaling Technology (2 mentions) Anti hsp60 sc 1722, by Santa Cruz Biotechnology (2 mentions)

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ECL solution (123 citations)

2 protocols using enhanced chemiluminiscent ecl solution

1

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies: anti-phospho-Erk (#9101), anti-Met (#8198), anti-phospho-Met (Tyr1234/1235) (#3077), anti-phospho-PLCγ1 (Tyr783) (#2821), from Cell Signaling Technologies, anti-HSP60 (sc-1722) from Santa Cruz Biotechnology. Cell lysates were prepared in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific). Equal amounts of protein, as measured by bicinchoninic (BCA) protein assay, were resolved in 4-12% Bis-Tris NuPage gradient gels (Life Technologies) and transferred electrophoretically to a polyvinylidene difluoride 0.45 μM membrane. Membranes were blocked in 5% non-fat milk in Tris-buffered saline and Tween 20 (TBST) before being incubated overnight at 4° C with the primary antibodies (1:1000 dilution in 5% non-fat milk in TBST). Signal detection was achieved by incubation of the membrane in enhanced chemiluminiscent (ECL) solution (Millipore) and autoradiography film exposure. The full blots are shown in Supplementary Figs. 8 and 9.
Yeh I., Botton T., Talevich E., Shain A.H., Sparatta A.J., de la Fouchardiere A., Mully T.W., North J.P., Garrido M.C., Gagnon A., Vemula S.S., McCalmont T.H., LeBoit P.E, & Bastian B.C. (2015). Activating MET Kinase Rearrangements in Melanoma and Spitz Tumors. Nature communications, 6, 7174.
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2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies: anti-phospho-Erk (#9101), anti-Met (#8198), anti-phospho-Met (Tyr1234/1235) (#3077), anti-phospho-PLCγ1 (Tyr783) (#2821), from Cell Signaling Technologies, anti-HSP60 (sc-1722) from Santa Cruz Biotechnology. Cell lysates were prepared in RIPA buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Scientific). Equal amounts of protein, as measured by bicinchoninic (BCA) protein assay, were resolved in 4-12% Bis-Tris NuPage gradient gels (Life Technologies) and transferred electrophoretically to a polyvinylidene difluoride 0.45 μM membrane. Membranes were blocked in 5% non-fat milk in Tris-buffered saline and Tween 20 (TBST) before being incubated overnight at 4° C with the primary antibodies (1:1000 dilution in 5% non-fat milk in TBST). Signal detection was achieved by incubation of the membrane in enhanced chemiluminiscent (ECL) solution (Millipore) and autoradiography film exposure. The full blots are shown in Supplementary Figs. 8 and 9.
Yeh I., Botton T., Talevich E., Shain A.H., Sparatta A.J., de la Fouchardiere A., Mully T.W., North J.P., Garrido M.C., Gagnon A., Vemula S.S., McCalmont T.H., LeBoit P.E, & Bastian B.C. (2015). Activating MET Kinase Rearrangements in Melanoma and Spitz Tumors. Nature communications, 6, 7174.
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