515dcxru

Manufactured by Chroma Technology

Sourced in United States

The 515dcxru is a lab equipment product. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Bx51wi microscope, by Olympus (3 mentions) 700dcxxr, by Chroma Technology (3 mentions)

3 protocols using 515dcxru

Optogenetic Control of Neuron Activity

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Cholinergic terminals expressing oChIEF-tdTomato were stimulated by blue light and interneurons expressing Arch-GFP were hyperpolarized by yellow light. Both light paths were transmitted through the epi-illumination light path of an Olympus BX51WI microscope and a 10× water immersion objective (0.3 NA). Blue light flashes (1 ms in duration) and yellow light pulses (4 s in duration) were generated from light-emitting diodes (LEDs) (UHP-microscope-LED-460 or UHP-T-LED-White filtered by an HQ 575/50x excitation filter, respectively, Prizmatix Modiin-Ilite, Givat Shmuel, Israel). Blue or yellow light exiting the LEDs were reflected or passed through a dichroic mirror (515dcxru, Chroma Technology, Bellows Falls, VT, USA) and were focused into the epi-illumination light path of the Olympus BX51WI microscope and back aperture of the 10x water immersion objective (0.3 NA) using an optiblock beam combiner (Prizmatix) and a dichroic mirror (700dcxxr, Chroma Technology, Bellows Falls, VT, USA) in the filter turret.

Bell L.A., Bell K.A, & McQuiston A.R. (2015). Acetylcholine release in mouse hippocampal CA1 preferentially activates inhibitory-selective interneurons via α4β2* nicotinic receptor activation. Frontiers in Cellular Neuroscience, 9, 115.

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Optogenetic Stimulation of mPFC Terminals

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mPFC terminals expressing oChIEF-tdtomato were stimulated by a train of 20 blue light pulses (1–2 ms in duration) delivered at 20 Hz frequency (20X20 Hz protocol). The light pulses were generated from a light emitting diode (LED) (UHP-microscope-LED-460, Prizmatix Modiin-Ilite, Givat Shmuel, Israel). Blue light exiting the LED were reflected by a dichroic mirror (515dcxru, Chroma Technology, Bellows Falls, VT, USA) using an optiblock beam combiner (Prizmatix) and were focused into the epi-illumination light path of an Olympus BX51WI microscope and back aperture of a 10x water immersion objective (0.3 NA) by a dichroic mirror (700dcxxr, Chroma Technology, Bellows Falls, VT, USA) in the filter turret.

Goswamee P., Rice R., Leggett E., Zhang F., Manicka S., Porter J.H, & McQuiston A.R. (2022). Effects of subanesthetic ketamine and (2R,6R) hydroxynorketamine on working memory and synaptic transmission in the nucleus reuniens in mice. Neuropharmacology, 208, 108965.

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Optogenetic Stimulation of Reuniens Neurons

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Reuniens terminals expressing oChIEF-tdtomato were stimulated by a train of 20 blue light pulses (1 ms in duration) delivered at 20 Hz frequency (20 × 20 Hz protocol). The light pulses were generated from a light-emitting diode (LED) (UHP-microscope-LED-460, Prizmatix Modiin Ilite, Givat Shmuel, Israel). Blue light exiting the LED was reflected by a dichroic mirror (515dcxru, Chroma Technology, Bellows Falls, VT, United States) using an optiblock beam combiner (Prizmatix) and focused into the epi-illumination light path of an Olympus BX51WI microscope and back aperture of a 10× water immersion objective (0.3 NA) by a dichroic mirror (700dcxxr, Chroma Technology, Bellows Falls, VT, United States) in the filter turret. The optogenetic parameters were kept constant for all experiments. To excite Jaws, an orange-light pulse was generated using the UHP-TLED-White light-emitting diode (LED) (Prizmatix Modiin-Ilite, Givat Shmuel, Israel). The white light exiting the LED was filtered by a HQ 605/50× filter (Chroma Technology).

Goswamee P., Leggett E, & McQuiston A.R. (2021). Nucleus Reuniens Afferents in Hippocampus Modulate CA1 Network Function via Monosynaptic Excitation and Polysynaptic Inhibition. Frontiers in Cellular Neuroscience, 15, 660897.

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