For histopathological examination, standard staining consisting of haematoxylin and eosin staining was performed. In addition, immunohistochemical workup of lymph node tissue was performed according to the underlying tumour type on an automated platform (Ventana Benchmark Ultra, Ventana Medical Systems, AZ, USA) adherent to manufacturer's protocols as follows: Melan-A (Clone: A103, ready to use kit; Ventana Medical Systems) and MITF (Clone: D5, 1:100; Agilent Dako, CA, USA) for melanoma and CK20 (Clone: Ks20.8, 1:1000; Agilent Dako) and Synaptophysin (Clone: MRQ-40, ready to use kit; Ventana Medical Systems) for Merkel cell carcinoma. Histopathologic evaluation of the lymph nodes was performed by an experienced pathologist from the Institute of Pathology or by an experienced dermatopathologist from the Department of Dermatology, Essen University Hospital. Histopathological lymph node specimens were digitised with the Aperio AT2 whole-slide scanner (Leica, Wetzlar, Germany) at 20× resolution.
At2 whole slide scanner
Manufactured by Leica camera
Sourced in Germany
The Leica AT2 whole-slide scanner is a device designed for digitizing and capturing high-resolution images of microscope slides. It serves as a tool for scanning and converting physical slide samples into digital format for further analysis and examination.
Automatically generated - may contain errors
4 protocols using at2 whole slide scanner
1
Lymph Node Histopathology Examination
2
SARS-CoV-2 Histopathology in Mice
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Mice were infected with USA-WA1/2020 and sacrificed 3-dpi. Tissues were fixed in 10% buffered formalin and processed through graded ethanol and xylene solutions and then embedded in paraffin with a Leica Peloris automated processor. Five-micron sections were deparaffinized and stained with hematoxylin and eosin on a Leica ST5020 automated histochemical stainer. The slides were scanned at 40X on a Leica AT2 whole slide scanner.
3
Tissue Fixation and Histological Staining
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Tissues were immersion-fixed in 10% neutral buffered formalin for 72 hours at room temperature and then processed through graded ethanol and xylene and into paraffin in a Leica Peloris automated processor. Five-micrometer, paraffin-embedded sections were deparaffinized and stained with hematoxylin (Leica, 3801575) and eosin (Leica, 3801619) on a Leica ST5020 automated histochemical strainer. Slides were scanned at original magnification 40× on a Leica AT2 whole-slide scanner and images transferred to the NYU Omero web-accessible image database.
4
Histological Analysis of SARS-CoV-2 Lungs
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The lungs of SARS-CoV-2-infected mice were harvested 3-dpi. The tissue was fixed in 10% neutral buffered formalin for 72 h at room temperature and then processed through graded ethanol, xylene, and into paraffin in a Leica Peloris automated processor. Then, 5-μm paraffin-embedded sections were deparaffinized and stained with hematoxylin (Leica, 3801575) and eosin (Leica, 3801619) on a Leica ST5020 automated histochemical strainer. Slides were scanned at 40× magnification on a Leica AT2 whole slide scanner, and the images were transferred to the NYULH Omero web-accessible image database.
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