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Ab2386

Manufactured by ABclonal
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Ab2386 is a laboratory equipment product manufactured by ABclonal. It is designed to perform a specific function within the laboratory setting. The detailed technical specifications and intended use of this product are not available for an unbiased and factual description without further interpretation.

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Lab products found in correlation

Iseq00010, by Merck Group (2 mentions) Ab64771, by Abcam (2 mentions) Mouse anti human collagen type 1, by Proteintech (2 mentions) Rabbit anti human tfrc, by ZenBio (2 mentions) Mouse anti human rhoa, by Santa Cruz Biotechnology (2 mentions) Mouse anti human iga antibody, by Southern Biotech (2 mentions) Rabbit anti human β tubulin, by ZenBio (2 mentions) Ecl plus detecting system, by GE Healthcare (2 mentions) Rabbit anti human gapdh, by Bioworld Technology (2 mentions)

2 protocols using ab2386

1

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
Zhong Z., Li Z., Li Y., Jiang L., Kong Q., Chen W, & Feng S. (2023). RhoA vesicle trafficking–mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy. JCI Insight, 8(19), e160374.
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2

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
Zhong Z., Li Z., Li Y., Jiang L., Kong Q., Chen W, & Feng S. (2023). RhoA vesicle trafficking–mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy. JCI Insight, 8(19), e160374.
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