MCSCs and MB49 cells were harvested separately, dissociated and labeled with fluorescein isothiocyanate (FITC) mouse antiCD44 (Miltenyi Biotec, Bergisch Gladbach, Germany) and phycoerythrin (PE) mouse anti-prominin-1 (Miltenyi Biotec). FITC rat IgG2b κ isotype control (eBioscience, San Diego, CA, USA) and PE rat IgG1 κ isotype control (eBioscience) were used as the negative control. The ratio of CD133+CD44+ cells was evaluated using a BD FACSAria cell sorter (Becton-Dickinson, San Jose, CA, USA).
Pe rat igg1 κ isotype control
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PE rat IgG1 κ isotype control is a laboratory reagent used as a control in flow cytometry experiments. It is a conjugate of the phycoerythrin (PE) fluorescent dye and a rat immunoglobulin G1 (IgG1) antibody with a kappa light chain. This reagent serves as a negative control to establish background fluorescence levels when analyzing cell samples.
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Lab products found in correlation
3 protocols using pe rat igg1 κ isotype control
1
Isolation and Identification of Cancer Stem Cells
2
Enrichment and Identification of CSCs
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The MB49, EJ and 5637 cells and their relevant CSCs were harvested respectively. They were dissociated in autoMACS running buffer (Miltenyi Biotec, Bergisch Gladbach, Germany), labeled with FITC antiCD44 (Miltenyi Biotec) and PE anti-prominin-1 (Miltenyi Biotec), incubated at 4°C for 20 minutes, and washed twice with PBS. The FITC rat IgG2bκ isotype control (eBioscience) and the PE rat IgG1κ isotype control (eBioscience) were used as a control. The portion of CD133+CD44+ cells was calculated using a BD FACSAria cell sorter (Becton-Dickinson, San Jose, California).
3
CD44+CD133+ Cell Identification
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The MB49, EJ, and SK-OV-3 cells and their trypsin-resistant cells were harvested, respectively. They were dissociated in autoMACS running buffer (Miltenyi Biotec, Bergisch Gladbach, Germany), labeled with PE antiprominin-1 (Miltenyi Biotec) and FITC anti-CD44 (Miltenyi Biotec), incubated and washed with PBS 2 times. The PE rat IgG1 κ isotype control (eBioscience) and the FITC rat IgG2b κ isotype control (eBioscience) were used as a control. The portion of CD44 + CD133 + cells was calculated using a BD FACSAria cell sorter (Becton-Dickinson, San Jose, California).
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