The human normal mammary epithelial cell line MCF-10A, the BC cell lines MDA-MB-231 and MCF7, and the embryonic kidney cell line 293T were purchased from the American Type Culture Collection. Cells were grown in Dulbecco's modified Eagle medium (DMEM; Biological Industries) supplemented with 10% Hyclone fetal bovine serum (FBS; Cytiva) at 37˚C in an atmosphere of 5% CO2.
Hyclone fetal bovine serum fbs
Manufactured by Cytiva
Sourced in Japan
Hyclone fetal bovine serum (FBS) is a high-quality cell culture supplement derived from the blood of bovine fetuses. It provides essential growth factors, proteins, and other nutrients required for the optimal growth and maintenance of a wide range of cell lines in in vitro cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Fungizone, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
L glutamine and phenol red, by Fujifilm (1 mentions)
Hmsc bm, by PromoCell (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
2 protocols using hyclone fetal bovine serum fbs
1
Cell Culture of Mammary and Kidney Lines
2
Human Bone Marrow Stromal Cell Culture
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Commercially available human BMSCs were obtained (hMSC-BM; PromoCell, Heidelberg, Germany; C-12974, lot number: 412Z022.4) as described previously.2 BMSCs were cultured with a complete culture medium, namely, Dulbecco's modified Eagle's medium (with low glucose levels; 2 mg/mL) containing L-glutamine and phenol red (DMEM; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), supplemented with 20% HyClone fetal bovine serum (FBS; Cytiva, Tokyo, Japan), 1% penicillin/streptomycin, 1·25 mg/mL fungizone (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), 1% HEPES (Life Technologies, Thermo Fisher Scientific), and 0·1% bFGF (Kaken Pharmaceutical Co., Ltd., Tokyo, Japan) in a humidified atmosphere (20% O2, 5% CO2, 37°C) according to the manufacturer's instructions. The medium was replaced twice per week, and BMSCs from the fourth passage process were used (six passages in total similar to that with RECs).
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