Multiscreen apparatus

Manufactured by Bio-Rad

The Multiscreen apparatus is a laboratory equipment used for filtration and separation procedures. It facilitates the efficient processing of multiple samples simultaneously. The device is designed to meet the needs of various applications in the field of biochemistry and molecular biology.

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Lab products found in correlation

Ovalbumin (ova), by Merck Group (1 mentions) Ecl substrate, by Merck Group (1 mentions) Horseradish peroxidase conjugated anti human igg, by Merck Group (1 mentions) Versadoc 4000 mp, by Bio-Rad (1 mentions)

Spelling variants of this product

Mini-PROTEAN II Multiscreen Apparatus (25 citations)

2 protocols using multiscreen apparatus

Quantitative Serum Antibody Evaluation

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10mg/ml b-galactosidase protein (Roche) or 3.3 mg/ml ovalbumin (Sigma-Aldrich)
were emulsified 1:1 with CFA. Each mouse received 4×50 ul emulsate. Three weeks
later, mice were bled and serum was analyzed by Western blot using a multiscreen apparatus
(Biorad). Density was compared between bands from samples and a known concentration of
monoclonal b-gal antibody (Clone DC1 4C7, Thermo Scientific) using ImageJ64 software.

Blachère N.E., Orange D.E., Santomasso B.D., Doerner J., Foo P.K., Herre M., Fak J., Monette S., Gantman E.C., Frank M.O, & Darnell R.B. (2014). T cells targeting a neuronal paraneoplastic antigen mediate tumor rejection and trigger CNS autoimmunity with humoral activation. European journal of immunology, 44(11), 3240-3251.

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Western Blotting for Immunodetection

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Western immunoblotting was performed as described previously [29] (link), with minor modifications. Briefly, proteins separated by SDS-PAGE (on 15% or 4–10% acrylamide gels) were electrically transferred onto nitrocellulose membranes and blocked in phosphate buffered saline, pH 7.4, 0.05% Tween 20 (PBS–T), containing 5% skim milk for 2 h. Membranes were then incubated with sera (1∶200 in PBS-T, 2% skim milk) for 1 h, either directly on membranes containing antigen preparations loaded in individual wells of 10-well gels, or by using a multiscreen apparatus (Bio-Rad) on membranes containing antigen preparations loaded in single-well gels. After washing, membranes were incubated with horseradish peroxidase conjugated anti-human IgG (Sigma) diluted 1∶500000 in 2% skim milk in PBS-T, developed with ECL substrate (Sigma), and digitized with VERSA DOC 4000 MP (Biorad).

Pagnozzi D., Biosa G., Addis M.F., Mastrandrea S., Masala G, & Uzzau S. (2014). An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid. PLoS ONE, 9(8), e104962.

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