2010 lab6

Manufactured by JEOL

Sourced in Japan

The JEOL 2010 LaB6 is a high-performance transmission electron microscope (TEM) equipped with a lanthanum hexaboride (LaB6) electron source. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of materials science applications.

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Lab products found in correlation

Cary 5000 uv vis nir spectrophotometer, by Agilent Technologies (2 mentions) Malvern zetasizer nano zs90, by Malvern Panalytical (2 mentions) Infinite m1000 pro, by Tecan (1 mentions) 2100 cryo, by JEOL (1 mentions) Genesys 10s uv vis spectrophotometer, by Thermo Fisher Scientific (1 mentions) Nanodrop 3300 fluorospectrometer, by Thermo Fisher Scientific (1 mentions)

4 protocols using 2010 lab6

Characterization of Mini Gold Nanorods

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UV-vis-NIR spectra were measured with a Cary 5000 UV-vis-NIR
spectrophotometer (Agilent Technologies). Transmission electron microscopy (TEM)
images of mini AuNRs were collected by a JEOL 2010 LaB6 or a JEOL 2100 Cryo
microscope (JEOL Ltd., Tokyo, Japan). Average lengths, widths, ARs, and shape
percent yields of mini AuNRs were determined by ImageJ software (the National
Institutes of Health). At least 300 particles were counted to determine the
dimensions of each batch of mini AuNRs. Inductively coupled plasma mass
spectrometry (ICP-MS) was used to determine the gold concentration of each
growth solution. A minimum of three measurements were taken for each batch of
mini AuNRs using a Thermo-Finnigan Element XR ICP-MS instrument (Thermo Fisher
Scientific).

Chang H.H, & Murphy C.J. (2018). Mini Gold Nanorods with Tunable Plasmonic Peaks beyond 1000 nm. Chemistry of materials : a publication of the American Chemical Society, 30(4), 1427-1435.

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Characterization of Gold Nanoparticles

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Hydrodynamic diameters and zeta potentials of AuNPs were determined with a ZetaPALS Particle Size and Zeta Potential Analyzer (Brookhaven, USA). UV−Vis−NIR spectra were measured with a Cary 5000 UV−Vis−NIR spectrophotometer (Agilent Technologies, USA). TEM of AuNPs was collected by a JEOL 2010 LaB6 or a JEOL 2100 Cryo electron microscope (JEOL Ltd., Japan). Average core diameters of AuNPs were determined by ImageJ software (National Institutes of Health, USA). At least 300 particles were counted to determine the diameters of AuNPs. The fluorescence intensity of the fluorescamine assay was determined by Tecan Infinite M1000 PRO multimode reader (Tecan Trading AG, Switzerland).

Sinclair W.E., Chang H.H., Dan A., Kenis P.J., Murphy C.J, & Leckband D.E. (2020). Gold nanoparticles disrupt actin organization and pulmonary endothelial barriers. Scientific Reports, 10, 13320.

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Characterizing Carbon Nanoparticle Properties

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The hydrodynamic diameters and surface charges of CNPs were determined by a Malvern Zetasizer Nano ZS90 (Malvern Instruments Ltd., Worcestershire, UK). The averaged hydrodynamic diameter was obtained from the peak values of the number distribution with 3 ≤ n ≤ 6 except only two measures available for CNP-PEG 20k and results were reported as mean ± standard deviation. Transmission electron microscopy (TEM) imaging was collected by JEOL 2010 LaB6 and JEOL 2100 Cryo (JEOL Ltd., Tokyo, Japan). Samples were deposited on 200-mesh Quantifoil®holey carbon grids (Structure Probe, Inc., PA, USA) to visualize their anhydrous morphology.

Misra S.K., Chang H.H., Mukherjee P., Tiwari S., Ohoka A, & Pan D. (2015). Regulating Biocompatibility of Carbon Spheres via Defined Nanoscale Chemistry and a Careful Selection of Surface Functionalities. Scientific Reports, 5, 14986.

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Characterizing C3 Nanoparticle Properties

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Hydrodynamic diameters and zeta potential of C³ were determined by a Malvern Zetasizer Nano ZS90 (Malvern Instruments Ltd., Worcestershire, UK) using water as a solvent. The averaged hydrodynamic diameter was obtained from the peak values of the intensity-weighted distribution with n≥3 and results were reported as mean ± standard deviation. Micrographs of transmission electron microscopy (TEM) was collected by JEOL 2010 LaB6 (JEOL Ltd., Tokyo, Japan) at an acceleration voltage of 200 kV. Samples were deposited on 200-mesh Quantifoil holey carbon grids (Structure Probe, Inc., PA, USA) to visualize their anhydrous sizes and morphology. Ultraviolet-visible (UV-Vis) absorbance of C³ and that of β-carotene were recorded via GENESYS 10S UV-Vis Spectrophotometer (Thermo Scientific, MA, USA). Absorbance spectra were collected at an interval of 1 nm from 250–900 nm. The emission spectra of fluorescence measurements were obtained through NanoDrop 3300 Fluorospectrometer (Thermo Scientific, MA, USA). The excitation maximum was set to 365 nm and the wavelength covered an excitation range between 400 and 750 nm.

Misra S.K., Mukherjee P., Chang H.H., Tiwari S., Gryka M., Bhargava R, & Pan D. (2016). Multi-functionality Redefined with Colloidal Carotene Carbon Nanoparticles for Synchronized Chemical Imaging, Enriched Cellular Uptake and Therapy. Scientific Reports, 6, 29299.

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