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Cytopeia s influx

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The BD Cytopeia's Influx is a high-performance cell sorter designed for advanced flow cytometry applications. It features a compact and modular design, allowing for customization to meet specific research needs. The Influx offers precision sorting capabilities, supporting a wide range of sample types and processing requirements.

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Lab products found in correlation

Lsrii fortessa, by BD (2 mentions) Fc500, by Beckman Coulter (2 mentions)

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Cytopeia Influx

2 protocols using cytopeia s influx

1

Flow Cytometry for Cell Characterization

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody staining for cell surface markers and flow cytometric analysis of the staining and EGFP signals were previously described24 (link). Intracellular Foxp3 transcription factor staining was performed as described23 (link). For the intracellular cytokine staining, cells were stimulated in culture with PMA/inomycin for 2–4 hours in presence of brefeldin A first and then stained similarly as for the intracellular transcription factor staining. Cells stained with a single antibody were used to calibrate signal output and compensation. Staining with isotype control antibodies was used to set gating for corresponding antigen-specific antibody staining. Flow cytometric analyses were performed on FC500 (Beckman Coulter) or BD Fortessa LSRII (BD Biosciences, San Jose, CA). Data were analyzed with FlowJo software (TreeStar, Ashland, OR). Cell gating is indicated in figures. Cell sorting was performed using BD Cytopeia’s Influx (BD Biosciences).
Yang J., Hu S., Zhao L., Kaplan D.H., Perdew G.H, & Xiong N. (2015). Selective programming of CCR10+ innate lymphoid cells in skin-draining lymph nodes for cutaneous homeostatic regulation. Nature immunology, 17(1), 48-56.
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2

Flow Cytometry for Cell Characterization

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody staining for cell surface markers and flow cytometric analysis of the staining and EGFP signals were previously described24 (link). Intracellular Foxp3 transcription factor staining was performed as described23 (link). For the intracellular cytokine staining, cells were stimulated in culture with PMA/inomycin for 2–4 hours in presence of brefeldin A first and then stained similarly as for the intracellular transcription factor staining. Cells stained with a single antibody were used to calibrate signal output and compensation. Staining with isotype control antibodies was used to set gating for corresponding antigen-specific antibody staining. Flow cytometric analyses were performed on FC500 (Beckman Coulter) or BD Fortessa LSRII (BD Biosciences, San Jose, CA). Data were analyzed with FlowJo software (TreeStar, Ashland, OR). Cell gating is indicated in figures. Cell sorting was performed using BD Cytopeia’s Influx (BD Biosciences).
Yang J., Hu S., Zhao L., Kaplan D.H., Perdew G.H, & Xiong N. (2015). Selective programming of CCR10+ innate lymphoid cells in skin-draining lymph nodes for cutaneous homeostatic regulation. Nature immunology, 17(1), 48-56.
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