Rabbit anti phospho cortactin

Manufactured by Cell Signaling Technology

Rabbit anti-phospho-Cortactin is a primary antibody that specifically recognizes the phosphorylated form of the Cortactin protein. Cortactin is an actin-binding protein involved in the regulation of the actin cytoskeleton and cell motility. This antibody can be used to detect and quantify the phosphorylation state of Cortactin in various cell and tissue samples.

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2 protocols using rabbit anti phospho cortactin

Immunoblotting and Immunoprecipitation Protocols

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Immunoblotting was performed as described (Jayabal et al., 2017 (link)). Immunoprecipitation was performed as described (Shiio and Eisenman, 2003 (link)). The following antibodies were used: rabbit anti-FLI-1 (ab15289, Abcam); rabbit anti-GDF6 (Novus, NBP 1-91934); rabbit anti-caspase 3 (9665, Cell Signaling Technology); rabbit anti-PARP (sc-7150, Santa Cruz Biotechnology); mouse anti-CD99 (MS-1633, Lab Vision); sheep anti-CD99L2 (AF5185, R&D Systems); mouse anti-p21 (BD PharMingen, 556430); mouse anti-tubulin (Developmental Studies Hybridoma Bank); rabbit anti-GAPDH (sc-25778, Santa Cruz Biotechnology); rabbit anti-CSK (HPA028425, Atlas Antibodies); mouse anti-GST (MS-707-P0, Lab Vision); mouse anti-GFP (sc-9996, Santa Cruz Biotechnology); rabbit anti-actin (8457, Cell Signaling Technology); rabbit anti-Src (2123, Cell Signaling Technology); rabbit anti-phospho-Src (2101, Cell Signaling Technology); rabbit anti-phospho-Cortactin (4569, Cell Signaling Technology); rabbit anti-phospho-p130 Cas (4011, Cell Signaling Technology); rabbit anti-phospho-STAT3 (9145, Cell Signaling Technology); rabbit anti-phospho-Smad1/5 (9516, Cell Signaling Technology); rabbit anti-HA (3724, Cell Signaling Technology); and mouse anti-FLAG (F1804, Sigma-Aldrich).

Zhou F., Elzi D.J., Jayabal P., Ma X., Chiu Y.C., Chen Y., Blackman B., Weintraub S.T., Houghton P.J, & Shiio Y. (2020). GDF6-CD99 Signaling Regulates Src and Ewing Sarcoma Growth. Cell reports, 33(5), 108332.

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Immunoblotting and Immunoprecipitation Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

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