The human GC cell lines MKN28 and MKN45 were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) and were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) at 37 °C in 5% CO2 and 95% relative humidity. An shRNA construct targeting EIF5A2 clone in a lentivirus vector (pLVTHM-sh-EIF5A2, sh-EIF5A2), an EIF5A2 overexpression vector (PIRES2-EGFP-EIF5A2, ov-EIF5A2), and respective negative control (NC) vectors, sh-NC and ov-NC, were purchased from Shanghai GenePharma (Shanghai, China). MKN28 and MKN45 cells displaying stable EIF5A2 overexpression or silencing were selected and cultured in appropriate antibiotic-containing RPMI 1640 medium. The expression of EIF5A2 was verified by RT-qPCR and Western blotting, and then the cells were used for subsequent experiments.
Sh eif5a2
Manufactured by GenePharma
Sourced in China
Sh-EIF5A2 is a laboratory equipment product designed for gene silencing experiments. It functions as a short hairpin RNA (shRNA) that specifically targets the EIF5A2 gene, which is involved in cellular processes. The core function of Sh-EIF5A2 is to facilitate the knockdown of the EIF5A2 gene expression in research settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Ov nc, by GenePharma (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sh nc, by GenePharma (1 mentions)
Mkn 45, by National Collection of Authenticated Cell Cultures (1 mentions)
Mkn28, by National Collection of Authenticated Cell Cultures (1 mentions)
Qbc939, by American Type Culture Collection (1 mentions)
Hibepic, by American Type Culture Collection (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Tfk 1, by American Type Culture Collection (1 mentions)
Hucct1, by American Type Culture Collection (1 mentions)
Shrna nc, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
2 protocols using sh eif5a2
1
Modulating EIF5A2 Expression in Gastric Cancer Cells
2
Investigating eIF5A's Role in CCA Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCA cells (HuCCT1, TFK-1, KKU-452, KKU-100, and QBC939) and human intrahepatic biliary epithelial cells (HIBEpiC) were purchased from ATCC. The DMEM medium (Invitrogen, USA) containing 10% fetal bovine serum (Gibco, USA) was used for cells. The culture conditions were 37°C and 5% CO2 in an incubator.
The cells were inoculated into 6-well plates according to the density of 2.5 × 105/well. sh-eIF5A#1 (5′-GCATTACGTAAGAATGGCTTT-3′), sh-eIF5A#2 (5′-GCATTCAAGATGGTTACCTTT-3′), sh-eIF5A#3 (5′-GCCATGTAAGATCGTCGAGAT-3′), shRNA-NC (5′-TTCTCCGAACGTGTCACGT-3′), pcDNA, and pcDNA-eIF5A were obtained from GenePharma (Shanghai, China). As previous studies [23 (link)], after overnight of culture, the serum-free medium was replaced, and then transfection was carried out by lipofectamine 3000 (Invitrogen). After 6 h of culture, medium was replaced; then the cells were cultured for 24 h. Following that, subsequent experiments were performed.
The cells were inoculated into 6-well plates according to the density of 2.5 × 105/well. sh-eIF5A#1 (5′-GCATTACGTAAGAATGGCTTT-3′), sh-eIF5A#2 (5′-GCATTCAAGATGGTTACCTTT-3′), sh-eIF5A#3 (5′-GCCATGTAAGATCGTCGAGAT-3′), shRNA-NC (5′-TTCTCCGAACGTGTCACGT-3′), pcDNA, and pcDNA-eIF5A were obtained from GenePharma (Shanghai, China). As previous studies [23 (link)], after overnight of culture, the serum-free medium was replaced, and then transfection was carried out by lipofectamine 3000 (Invitrogen). After 6 h of culture, medium was replaced; then the cells were cultured for 24 h. Following that, subsequent experiments were performed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!