Immunoprecipitation and immunoblotting experiments were performed as previously described.34 (link) Antibodies against HA (1:2000; clone C29F4, cat # 3724S), β-actin (1:5000; clone 13E5, cat # 4970S), H3 (1:4000; clone 1B1B2, cat # 14269S) and RAD23A (1:1000; clone D7U7Z, cat # 24555) were purchased from Cell Signaling Technology, as was a non-specific isotype control IgG (clone DA1E, cat # 3900S). The FLAG antibody was from Sigma-Aldrich (1:4000; clone M2, cat # F1804). The Pol ι antibody was from Abnova (1:1000; clone M01, cat # H00011201-M01). Primary antibodies were detected with fluorescent secondary antibodies, and immunoblots quantified, as described previously.34 (link) For the microarray, the Pol ι antibody was detected with an Alexa Fluor 647-conjugated goat anti-mouse secondary antibody (Thermo Fisher Scientific cat #A-21240).
Rad23a
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
RAD23A is a protein involved in DNA repair. It plays a role in the nucleotide excision repair pathway, which is responsible for removing and replacing damaged DNA segments. RAD23A is a component of the damage recognition complex that initiates the repair process.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 647 conjugated goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
Clone da1e, by Cell Signaling Technology (1 mentions)
Clone 1b1b2, by Cell Signaling Technology (1 mentions)
Clone 13e5, by Cell Signaling Technology (1 mentions)
Clone m2, by Merck Group (1 mentions)
Clone m01, by Abnova (1 mentions)
Clone c29f4, by Cell Signaling Technology (1 mentions)
Flag antibody, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ab109123, by Abcam (1 mentions)
Lactacystin, by Peptide Institute (1 mentions)
Mg132, by Fujifilm (1 mentions)
Lamin b1 c 20, by Santa Cruz Biotechnology (1 mentions)
Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (1 mentions)
α tubulin, by Merck Group (1 mentions)
2 protocols using rad23a
1
Immunoprecipitation and Immunoblotting Protocol
2
Site-Directed Mutagenesis of PSMD14
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Site-directed mutagenesis of PSMD14 was performed with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA) and primer sequences described previously56 (link). MLN4924 (Sequoia Research Products, Pangbourne, UK), MG132 (Fujifilm Wako Pure Chemical), and lactacystin (Peptide Institute, Ibaraki, Osaka, Japan) were purchased from the indicated suppliers. Generation of antibodies against XPC69 (link), RAD23B30 (link), and PSMD457 (link) was described previously. The following antibodies were purchased from manufacturers: XPC (NB100-477, Novus Biologicals, Centennial, CO, USA), DDB2 (AF3297, R&D Systems, Minneapolis, MN, USA), CUL4A (14851-1-AP, Proteintech, Rosemont, IL, USA), multi-ubiquitin (FK2; D058-3, Medical & Biological Laboratories, Nagoya, Japan), mKO2 (PM051M, Medical & Biological Laboratories), PSMD14 (ab109123, abcam, Cambridge, UK), RAD23A (24555, Cell Signaling Technology, Danvers, MA, USA), α-tubulin (T5168, Merck), and lamin B1 (C-20, Santa Cruz Biotechnology, Santa Cruz, CA, USA).
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