Anti egfr mab

Formalin‐fixed patient‐derived tumor samples, spheroids and excised U87‐MGvIII tumors, either following irradiation or affibody‐based PIT, were embedded in paraffin, sectioned (5 μm‐thick slices) and mounted on microscope slides. Multiple sections were taken at regular intervals across each tumor, with sequential sections being stained with H&E (Leica biosystems, Buffalo Grove, IL), anti‐Ki67 mAb (1:400, Cell Signaling Technology, Danvers, Massachusetts) and anti‐EGFR mAb (1:400, Dako, Santa Clara, CA). Orthotopic tumors were snap‐frozen in OCT solution and sectioned (10 µm‐thick slices) and mounted on microscope slides before being fixed in ice‐cold acetone and imaged using a Typhoon™ FLA7000 scanner (ex. 635 nm, band filter 670 nm; GE Healthcare Life Sciences, Chicago, IL). Following fluorescence imaging, the slides underwent IHC by staining with H&E (Leica Biosystems, Buffalo Grove, IL), anti‐affibody IgG mAb (1:50, AffibodyAB, Stockholm, Sweden) and anti‐EGFR mAb (1:200, Dako, Santa Clara, CA).