The following microscopes were used: (1) Zeiss LSM 880 inverted laser scanning confocal microscope equipped with a plan-apochromatic 63× oil objective lens (Zeiss, NA = 1.4), 40× oil objective lens (Zeiss, NA = 1.3), 405 nm, 488 nm, and 561 nm laser lines, and ZEN software (Zeiss, Oberkochen, Germany). (2) Nikon Eclipse TiE inverted microscope equipped with a CFI Plan Apo Lambda 10× (Nikon Instruments, NA = 0.45) and a CFI Plan Apo VC 60× oil objective lens (Nikon Instruments, NA = 1.4), sCMOS camera (Zyla 4.2, Andor), SPECTRA X light engine illumination source (Lumencor, Beaverton, OR), and NIS-Elements software (Nikon Instruments, Tokyo, Japan). The experimental details for each imaging experiment are provided in the appropriate subsection in Materials and methods.
Spectra x light engine illumination source
Manufactured by Lumencor
Sourced in Japan
The SPECTRA X light engine is a multi-wavelength illumination source designed for use in microscopy and other scientific applications. It features a compact and modular design, allowing for the selection of multiple wavelengths from a single unit. The SPECTRA X provides stable and consistent illumination across a wide range of wavelengths, making it a versatile tool for researchers and scientists.
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2 protocols using spectra x light engine illumination source
1
Multimodal Microscopy for Biological Imaging
2
Multimodal Imaging of Cell Structures
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The following microscopes were used: (1) Zeiss LSM 880 inverted laser scanning confocal microscope equipped with a plan-apochromatic 63x oil objective lens (Zeiss, NA = 1.4), 40x oil objective lens (Zeiss, NA = 1.3), 405 nm, 488 nm and 561 nm laser lines, and ZEN software (Zeiss, Oberkochen, Germany). (2) Nikon Eclipse TiE inverted microscope equipped with a CFI Plan Apo Lambda 10x (Nikon Instruments, NA = 0.45) and a CFI Plan Apo VC 60x oil objective lens (Nikon Instruments, NA = 1.4), sCMOS camera (Zyla 4.2, Andor), SPECTRA X light engine illumination source (Lumencor, Beaverton, OR), and NIS-Elements software (Nikon Instruments, Tokyo, Japan). The experimental details for each imaging experiment are provided in the appropriate subsection in Materials and Methods.
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