CMT93 cells were cultured with DMEM containing 0.1% FBS and 2 μg/ml recombinant Postn for 72 hours. Cells were harvested with 0.25% trypsin and stained with FITC-conjugated anti-annexin V (Biolegend) and 7-AAD (Sigma Aldrich). The cells were analyzed with a FACSCanto II.
Fitc conjugated anti annexin 5
Manufactured by BioLegend
FITC-conjugated anti-annexin V is a fluorescently labeled antibody that binds to annexin V, a calcium-dependent phospholipid-binding protein. This product can be used to detect and quantify annexin V in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated anti annexin 5
1
Postn-induced Apoptosis in CMT93 Cells
2
RASF Apoptosis Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Following C3G treatment, trypsin was added to the RASF culture, and the cells were collected by centrifugation. The binding buffer was added to resuspend the cell pellet, and FITC-conjugated anti-Annexin V (BioLegend) antibody and PI-conjugated antibody (BioLegend) were then added to the suspended RASF cells in dark for 15 min. Apoptosis was measured by flow cytometry.
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