Estrone, methyl tert-butyl ether (MTBE), α-cyano-4-hydroxycinnamic acid (CHCA), dimethyl sulfoxide (DMSO) and letrozole were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol and acetic acid were obtained from Junsei Chemical (Tokyo, Japan). Girard’s reagent P (GP) was obtained from Tokyo Chemical Industry (Tokyo, Japan). Estrone-2,4,16,16-d4 was obtained from Cayman Chemical (Ann Arbor, MI). ReadyPrep proteomic-grade water was obtained from Bio-Rad Laboratories (Hercules, CA). Roswell Park Memorial Institute (RPMI-1640) medium, a phosphate-buffered saline (PBS) buffer, Hanks’ Balanced Salt solution (HBSS) and penicillin-streptomycin (Pen-Strep) were purchased from Gibco (Grand Island, NY). Fetal bovine serum (FBS) was obtained from the American Type Culture Collection (ATCC). Human normal sera were kindly provided by Prof. Jun Kyu Lee (Dongguk University Ilsan Hospital, Korea). The blood specimens are samples that were discarded under approved institutional review board protocols.
Readyprep proteomic grade water
Manufactured by Bio-Rad
Sourced in United States
ReadyPrep proteomic-grade water is a high-purity laboratory water used in proteomics research and applications. It is designed to meet the stringent requirements for proteomic sample preparation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Junsei (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Letrozole, by Merck Group (1 mentions)
Estrone, by Merck Group (1 mentions)
Penicillin streptomycin pen strep, by Thermo Fisher Scientific (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline buffer, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Acetonitrile, by Junsei (1 mentions)
Methyl iodide, by Merck Group (1 mentions)
Peptide n glycosidase f, by Roche (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Chloroform, by Junsei (1 mentions)
2 protocols using readyprep proteomic grade water
1
Estrogen Receptor Quantification Protocol
2
N-Glycan Release and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sodium hydroxide (beads, 20–40 mesh), 5-dihydroxybenzoic acid (DHB), dimethyl sulfoxide (DMSO), methyl iodide, and trifluoroAcetic acid (TFA) were purchased from Sigma-Aldrich (USA). Peptide N-glycosidase F (PNGase F) was obtained from Roche (Germany). Porous graphitic carbon (PGC) cartridge was purchased from Thermo Scientific (USA) and Microspin column was obtained from Harvard Apparatus (USA). Acetic acid, acetonitrile (ACN), and chloroform were purchased from Junsei (Japan). All aqueous solutions were prepared with ReadyPrep™ Proteomic Grade Water (Bio-Rad, USA).
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