Uplc ms platform

To identify changes of BA profiles after IT, 17 kinds of BAs, such as α-muricholic acid (α-MCA), β-muricholic acid (β-MCA), cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), hyodeoxycholic acid (HDCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA), glycoursodeoxycholic acid (GUDCA), lithocholic acid (LCA), tauro-α-muricholic acid (T-α-MCA), tauro-β-muricholic acid (T-β-MCA), taurocholic acid (TCA), turoursodeoxycholic acid (TDCA), taurohyodeoxycholic acid (THDCA) and tauroursodeoxycholic acid (TUDCA) were measured in samples.
All rats were fasted overnight and given 20% Intralipid fluid solution (Fresenius Kabi SSPC, Wuxi, P. R. China) orally at postoperative week 16. At 1 hour after feeding, blood was taken from the caudal veins. Quantitative detection of targeted BAs was performed on an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) platform (Waters, Milford, MA, USA). The procedure was described in previous reports 17 (link).

To identify the alteration in the bile acid profiles induced by biliary diversion, we measured and identified 18 bile acids in plasma samples collected from GK rats before surgery and 8 weeks postoperatively through targeted metabolomics analysis. At postoperative week 8, all rats were gavaged with 10% ENSURE® (1 ml/100 g body weight (31 (link))) after fasting for 12 h. Blood was then collected from the angular vein at 2 hours after gavage. Targeted quantitative detection of serum bile acid was performed on an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC−MS) platform (Waters Corporation, Milford, MA, USA) using the Waters ACQUITY UPLC I-class ULTRA high-performance liquid chromatography system combined with the Waters XEVO TQ-S tandem quadrupole mass spectrometry system. Data acquisition was carried out with MassLynx v 4.1 software (Waters Corporation, Milford, MA, USA).