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Cd45 bv421 clone hi30

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CD45-BV421 (clone HI30) is a fluorescently labeled antibody that binds to the CD45 cell surface antigen. CD45 is a protein tyrosine phosphatase expressed on the surface of all leukocytes. This antibody can be used to identify and differentiate various immune cell populations in flow cytometry applications.

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Lab products found in correlation

Cd3 af647 clone hit3a, by BioLegend (3 mentions) Cd19 percp clone hib19, by BioLegend (3 mentions) Facscanto 2, by BD (3 mentions) Cd45 bv421, by BioLegend (2 mentions) Cd14 af488 clone m5e2, by BioLegend (2 mentions) Cd56 percp cy5.5 clone 5 1h11, by BioLegend (2 mentions) Pan flavivirus antibody clone d1 4g2 4 15, by Merck Group (2 mentions) Cd16 af647 clone 3g8, by BioLegend (2 mentions) Lsr 3, by BD (2 mentions) Cd14 af488, by BioLegend (2 mentions) Cd14 bv785 clone m5e2, by BioLegend (1 mentions) Zombie aqua fixable viability dye, by BioLegend (1 mentions) Cd19 pe cy7 clone hib19, by BioLegend (1 mentions) Cytoflex v5 b5 r3 flow cytometer, by Beckman Coulter (1 mentions) Gamma immune, by Merck Group (1 mentions)

4 protocols using cd45 bv421 clone hi30

1

Multiparametric Flow Cytometry Analysis

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Cytometry results represent at least three biological replicates for each time point on long-term culture and were obtained from a combination of seven experiments with mixes of 10 independent healthy donors. Cells were cultured in a prestimulation medium with or without metabolic drugs and αKG during the first 4 d and then in the optimized prestimulation medium without inhibitor or αKG as described before. A sample of cells were collected at days 4, 7, 10, and 14 to be analyzed by flow cytometry on CytoFLEX V5-B5-R3 Flow Cytometer (Beckman Coulter). After a saturation step with Gamma Immune (dilution 1:2; Sigma-Aldrich), cells were labeled with antibodies detecting the following cell surface markers: CD133-APC (clone 7, dilution 1:20; BioLegend), CD19-PE Cy7 (clone HIB19, dilution 1:80; BioLegend), CD36-PerCP-Cy5.5 (clone 5–271, dilution 1:40; BioLegend), CD45-BV421 (clone HI30, dilution 1:80; BioLegend), CD15-APC-Fire 750 (clone W6D3, dilution 1:80; BioLegend), CD14-BV785 (clone M5E2, dilution 1:80; BioLegend), CD41-BV605 (clone HIP8, dilution 1:40; BioLegend), and Zombie Aqua fixable viability dye (dilution 1:200; BioLegend). For each experiment, compensation beads (ref. 130-097-900; Miltenyi) were used for positive monolabeling controls and unmarked cells for negative controls.
Racine L., Parmentier R., Niphadkar S., Chhun J., Martignoles J.A., Delhommeau F., Laxman S, & Paldi A. (2024). Metabolic adaptation pilots the differentiation of human hematopoietic cells. Life Science Alliance, 7(8), e202402747.
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2

PBMC Immunophenotyping and ZIKV Infection

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Surface staining of PBMCs were performed with the following antibodies: CD45-BV421 (clone HI30, Biolegend), CD14-AF488 (clone M5E2, Biolegend), CD56-PerCP-Cy5.5 (clone 5.1H11, Biolegend), CD3-AF647 (clone HIT3a, Biolegend) and CD19-PerCP (clone HIB19, Biolegend). PBMCs specimens were either sorted based on surface markers expression using BD LSR III (BD) or proceed on to intracellular ZIKV staining for detection of ZIKV-infected cells. Indirect intracellular ZIKV staining was performed using pan flavivirus antibody (clone D1-4G2-4-15, EMD Millipore), followed by fluorescence-conjugated secondary antibody. For profiling of monocyte subsets, separate surface staining for CD45-BV421, CD14-AF488 and CD16-AF647 (clone 3G8, Biolegend) were performed on PBMCs. All fluorescence-conjugated antibodies were purchased from Biolegend. FACS acquisitions were performed on BD FACSCanto II (BD), using BD FACSDIVA software. All FACS data was analyzed using FlowJo software.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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3

Multicolor Flow Cytometry Analysis

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Surface staining of indicated subsets was performed with the following antibodies: CD45-BV421 (clone HI30; Biolegend), CD14-Alexa Fluor 488 (AF488) (clone 63D3; Biolegend), CD56-peridinin chlorophyll protein (PerCP)-Cy5.5 (clone 5.1H11; Biolegend), CD3-AF647 (clone HIT3a; Biolegend), and CD19-PerCP (clone HIB19; Biolegend). ORF8-Flag staining was performed using phycoerythrin (PE)-conjugated fluorescence-activated cell sorter (FACS) antibody (Biolegend). FACS acquisitions were performed on BD FACSCanto II (BD), using BD FACSDIVA software. All FACS data were analyzed using FlowJo software.
Wu X., Xia T., Shin W.J., Yu K.M., Jung W., Herrmann A., Foo S.S., Chen W., Zhang P., Lee J.S., Poo H., Comhair S.A., Jehi L., Choi Y.K., Ensser A, & Jung J.U. (2022). Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8. mBio, 13(2), e00402-22.
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4

PBMC Immunophenotyping and ZIKV Infection

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Surface staining of PBMCs were performed with the following antibodies: CD45-BV421 (clone HI30, Biolegend), CD14-AF488 (clone M5E2, Biolegend), CD56-PerCP-Cy5.5 (clone 5.1H11, Biolegend), CD3-AF647 (clone HIT3a, Biolegend) and CD19-PerCP (clone HIB19, Biolegend). PBMCs specimens were either sorted based on surface markers expression using BD LSR III (BD) or proceed on to intracellular ZIKV staining for detection of ZIKV-infected cells. Indirect intracellular ZIKV staining was performed using pan flavivirus antibody (clone D1-4G2-4-15, EMD Millipore), followed by fluorescence-conjugated secondary antibody. For profiling of monocyte subsets, separate surface staining for CD45-BV421, CD14-AF488 and CD16-AF647 (clone 3G8, Biolegend) were performed on PBMCs. All fluorescence-conjugated antibodies were purchased from Biolegend. FACS acquisitions were performed on BD FACSCanto II (BD), using BD FACSDIVA software. All FACS data was analyzed using FlowJo software.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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