These constructs were made as described above, but with a bridge oligonucleotide containing an inserted sequence recognized by the XhoI enzyme (New England Biolabs). The compliment to this restriction sequence was also added to ensure that this region was double stranded. Cleavage measurements were performed by adding enzyme to the loops (with final concentrations of 2.2 nM and 1,000 units/mL for the loops and enzyme, respectively) in the recommended buffer (New England Biolabs) and quenching the enzyme activity with 75 mM EDTA at various times at room temperature.
Recommended buffer
Manufactured by New England Biolabs
Recommended buffer is a high-performance, multi-purpose buffer solution designed for use in a wide range of molecular biology applications. It maintains stable pH and ionic conditions to support optimal enzymatic activity and sample integrity.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recommended buffer
1
Quantifying Restriction Enzyme Cleavage
2
Quantifying Restriction Enzyme Cleavage
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