Log In Sign up
The largest database of trusted experimental protocols

Collagenase crude type 1 a

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Collagenase crude type I A is an enzyme used for the dissociation and isolation of cells from various tissue types. It is a mixture of enzymes that break down the collagen in the extracellular matrix, allowing for the release of individual cells.

Automatically generated - may contain errors

Lab products found in correlation

Trypan blue, by Merck Group (2 mentions) Fetal calf serum (fcs), by Dutscher (2 mentions) Fluorescein isothiocyanate fitc dextran, by Merck Group (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Pge2 eia kit monoclonal, by Cayman Chemical (1 mentions) Protanal lf 10 60 ft, by FMC Biopolymer (1 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) Calcium chloride, by Merck Group (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Sodium citrate, by Merck Group (1 mentions) Live dead assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Collagenase A type I Collagenase type A Crude collagenase Collagenase type I Type collagenase Collagenase A Type A Collagenase I Collagenases I Type I

2 protocols using collagenase crude type 1 a

1

Hydrogel-based cell culture platform

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium alginate (Protanal™LF10/60FT) and hydroxypropyl methylcellulose (HPMC) (Methocel™E4M) were purchased, respectively, from FMC Biopolymer and Colorcon-Dow chemical (Bougival, France). Glycidoxypropyltrimethoxysilane (GPTMS) was obtained from Acros (Geel, Belgium). Hank's balanced sodium salt (HBSS), Dulbecco's modified eagle medium (DMEM) high glucose (4.5 g/L), phosphate-buffered salt (PBS) without Calcium chloride and magnesium chloride, penicillin/streptomycin, and trypsin/EDTA (0.05%/0.53 mM) were obtained from Invitrogen (Paisley, UK). Calcium chloride, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), olive oil, fluorescein isothiocyanate- (FITC-) dextrans, collagenase crude type I A, trypan blue, sodium citrate, and trichloroacetic acid (TCA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal calf serum (FCS) was purchased from Dominique Dutscher (Brumath, France). Live/Dead Viability/Cytotoxicity kit and Quant-iT PicoGreen dsDNA assay kit were, respectively, obtained from Molecular Probes (Leiden, The Netherlands) and Thermo Fisher (Waltham, MA, USA). PGE2 EIA Kit-Monoclonal and Human HGF Duo Set ELISA were purchased from Cayman Chemical and R&D Systems, respectively.
Hached F., Vinatier C., Pinta P.G., Hulin P., Le Visage C., Weiss P., Guicheux J., Billon-Chabaud A, & Grimandi G. (2017). Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International, 2017, 9303598.
+ Open protocol
+ Expand
2

HPMC-GPTMS Microfluidic Cellular Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydroxypropylmethylcellulose (HPMC) (MethocelTME4M) was purchased from Colorcon-Dow chemical (Bougival, France). Glycidoxypropyltrimethoxysilane (GPTMS) was obtained from Acros (Geel, Belgium). Hank’s Balanced Sodium Salt (HBSS), Dulbecco’s Modified Eagle Medium high glucose (4.5 g/L) (DMEM), phosphate buffered salt (PBS) without calcium chloride and magnesium chloride, penicillin/streptomycin, and trypsin/EDTA (0.05%/0.53 mM) were obtained from Invitrogen (Paisley, UK). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), olive oil, fluorescein isothiocyanate (FITC)-dextrans, collagenase crude type I A, and trypan blue were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal calf serum (FCS) was purchased from Dominique Dutscher (Brumath, France). The Live/Dead assay kit was obtained from Molecular Probes (Leiden, The Netherlands). Twelve-well plates (ref. 3512) were purchased from Corning (Boulogne Billancourt, France).
PolyEtherEtherKetone (PEEK) tubing for millifluidics were purchased from CIL, Cluzeau Info Labo (Sainte Foy la Grande, France).
Nativel F., Renard D., Hached F., Pinta P.G., D’Arros C., Weiss P., Le Visage C., Guicheux J., Billon-Chabaud A, & Grimandi G. (2018). Application of Millifluidics to Encapsulate and Support Viable Human Mesenchymal Stem Cells in a Polysaccharide Hydrogel. International Journal of Molecular Sciences, 19(7), 1952.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!