Migration assays were performed using transwell inserts with 8 μm pore size filters (Corning, NY, USA) in a 24 well plate. MDA-MB-231, MDA-MB-436, and Hs578T cells were serum starved overnight in corresponding appropriate media. On the following day, 5 × 104 tumor cells were mixed with drug treatments and placed in the top chamber of the insert in 300 μL of serum free media. Media containing serum was placed in the bottom chamber. After 24 hours, cells on the top of the filter were removed using a cotton swab and the cells on the bottom of the filter and well bottom were stained in a 0.5% (w/v) solution of crystal violet (Sigma) in 60% PBS and 40% ethanol. The inserts and well bottoms were rinsed with PBS and the number of migrated cells/field was quantified using Image J. At least four independent fields were quantified in each experiment.
Transwell inserts with 8 μm pore size filters
Manufactured by Corning
Sourced in United States
Transwell inserts with 8 μm pore size filters are a type of lab equipment used for cell migration and invasion assays. The inserts feature a semi-permeable membrane with a pore size of 8 micrometers, which allows cells to migrate through the membrane.
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Lab products found in correlation
2 protocols using transwell inserts with 8 μm pore size filters
1
Transwell Assay for Cell Migration
2
Transwell Migration Assay Protocol
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Transwell migration assays were performed as described previously.30 Briefly, Transwell inserts with 8‐μm pore size filters (Corning) pre‐coated on both sides with fibronectin were inserted into 24‐well plates. DMEM containing 10% FBS was added to the lower chamber and a cell suspension (5 × 104 cells) was placed in the upper chamber. The plates were incubated at 37°C with 5% CO2 for 6 h. After incubation, the cells that had migrated to the lower side were stained with 0.5% crystal violet solution or Giemsa solution and counted using a light microscope at ×200 magnification. Values represent averages from 5 fields.
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