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2 protocols using anti marco

1

Immunofluorescence Staining Antibody Protocol

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Primary antibodies for mouse antigens: anti-eGFP (Abcam, clone 7.1 and 13.1), anti-MAdCAM-1 (eBioscience, MECA-367), anti-LYVE-1 (ReliaTech, 103-PA50), anti-PD-L1 (BioLegend, 10F.9G2), anti-Marco (Bio-Rad, ED31), and anti-B220/CD45R (ebiosciences, RA3-6B2). Primary antibodies for human antigens in fresh frozen tissue: anti-PROX-1 (R and D, AF2727), anti-LYVE1 (Reliatech, 102-PA50), anti-CLEC4M (R and D, MAB162), anti-Marco (Sigma, HPA063793), and anti-CD36 (Abcam, ab17044). Primary antibodies for human antigens in FFPE tissue: anti-PDPN (Dako, clone D2-40), anti-LYVE-1 (RnD Systems), anti-CCL21 (RnD Systems), anti-CD36 (HPA, Sigma-Aldrich), anti-STAB2 (HPA, Sigma-Aldrich), and anti-Claudin-5 (Invitrogen, clone 4C3C2). Secondary antibodies: donkey-anti-chicken AF488 (Jackson ImmunoResearch), donkey anti-rabbit Cy3 (Jackson ImmunoResearch), donkey anti-rabbit AF647 (Invitrogen), goat anti-rabbit AF546 (Invitrogen), donkey anti-rabbit AF555 (Invitrogen), donkey anti-mouse Cy3 (Jackson ImmunoResearch), donkey anti-mouse AF647 (Invitrogen), donkey anti-mouse AF594 (Invitrogen), donkey anti-goat AF488, AF555 and AF594 (Invitrogen), bovine anti-goat AF647 (Jackson ImmunoResearch), and donkey anti-rat AF488 and AF647 (Jackson ImmunoResearch).
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2

Comprehensive Immunostaining Antibody Panel

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Primary antibodies for mouse antigens: anti-eGFP (Abcam, clone 7.1 and 13.1), anti-MAdCAM-1 (eBioscience, MECA-367), anti-LYVE-1 (ReliaTech, 103-PA50), anti-PD-L1 (BioLegend, 10F.9G2), anti-Marco (Bio-Rad, ED31), and anti-B220/CD45R (ebiosciences, RA3-6B2). Primary antibodies for human antigens in fresh frozen tissue: anti-PROX-1 (R and D, AF2727), anti-LYVE1 (Reliatech, 102-PA50), anti-CLEC4M (R and D, MAB162), anti-Marco (Sigma, HPA063793), and anti-CD36 (Abcam, ab17044). Primary antibodies for human antigens in FFPE tissue: anti-PDPN (Dako, clone D2-40), anti-LYVE-1 (RnD Systems), anti-CCL21 (RnD Systems), anti-CD36 (HPA, Sigma-Aldrich), anti-STAB2 (HPA, Sigma-Aldrich), and anti-Claudin-5 (Invitrogen, clone 4C3C2). Secondary antibodies: donkey-anti-chicken AF488 (Jackson ImmunoResearch), donkey anti-rabbit Cy3 (Jackson ImmunoResearch), donkey anti-rabbit AF647 (Invitrogen), goat anti-rabbit AF546 (Invitrogen), donkey anti-rabbit AF555 (Invitrogen), donkey anti-mouse Cy3 (Jackson ImmunoResearch), donkey anti-mouse AF647 (Invitrogen), donkey anti-mouse AF594 (Invitrogen), donkey anti-goat AF488, AF555 and AF594 (Invitrogen), bovine anti-goat AF647 (Jackson ImmunoResearch), and donkey anti-rat AF488 and AF647 (Jackson ImmunoResearch).
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