Daytona plus rx series analyzer

Before laboratory analysis, retrospectively identified frozen plasma samples were thawed at room temperature, centrifuged for 2 min at 4000 rpm, and then treated as per the individualized assay kit instructions. The Randox Daytona Plus RX series analyzer (Randox Laboratories Ltd.®, Crumlin, UK) was used for in vitro determination of β-hydroxybutyrate ([β-OHB] RB4067). C-peptide was quantified using Invitron's Molecular Light Technology™ chemiluminescence assay (IV2-004; Invitron Ltd.®, Monmouth, UK). Proinsulin was quantified using Invitron's Molecular Light Technology™ chemiluminescence intact assay (IV2-002, Invitron Ltd.). Glucagon was quantified using the R&D Systems® (R&D Systems, Inc., Minneapolis, MN) Glucagon Quantikine ELISA Kit (product no. DGCG0). The Eagle Biosciences (Eagle Biosciences, Inc., Amherst, NH) BI-CAT Epinephrine & Norepinephrine ELISA Assay Kit (product no. ECT31-K02) was used for the determination of catecholamines. Trial-day venous BG and blood lactate (BLa) concentrations were measured via the fully enzymatic-amperometric method (Biosen C-Line; EKF-diagnostic GmbH, Barleben, Germany).