Faecal samples were collected using a guanidine thiocyanate solution (Faeces Collection kit; Techno Suruga Lab, Shizuoka, Japan). After vigorous mixing, the samples were stored at 4 °C for a maximum of 7 days until DNA extraction. After homogenisation with lysis solution F (Nippon Gene, Tokyo, Japan), the genomic DNA was heated at 65 °C for 10 min and purified from the supernatants using the MPure Bacterial DNA Extraction Kit (MP Biomedicals, Solon, OH, USA) with MPure-12 system (MP Biomedicals). The purified DNA was quantified using Synergy LX (BioTek Instruments, Winooski, VT, USA) and QuantiFluor system (Promega, Madison, WI, USA).
Mpure 12 system
Manufactured by MP Biomedicals
Sourced in United States
The MPure-12 system is a compact and automated DNA/RNA purification platform. It utilizes magnetic bead-based technology to extract nucleic acids from a variety of sample types. The system can process up to 12 samples simultaneously, providing a streamlined and efficient nucleic acid isolation solution.
Automatically generated - may contain errors
2 protocols using mpure 12 system
1
Fecal DNA Extraction Protocol
2
Faecal DNA Extraction Protocol
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Faecal samples were collected using a guanidine thiocyanate solution (Faeces Collection kit; Techno Suruga Lab, Shizuoka, Japan). After vigorous mixing, the samples were stored at 4°C for a maximum of 7 days until DNA extraction. After homogenisation with lysis solution F (Nippon Gene, Tokyo, Japan), the genomic DNA was heated at 65°C for 10 min and puri ed from the supernatants using the MPure Bacterial DNA Extraction Kit (MP Biomedicals, Solon, OH, USA) with MPure-12 system (MP Biomedicals).
The puri ed DNA was quanti ed using Synergy LX (BioTek Instruments, Winooski, VT, USA) and QuantiFluor ® system (Promega, Madison, WI, USA).
The puri ed DNA was quanti ed using Synergy LX (BioTek Instruments, Winooski, VT, USA) and QuantiFluor ® system (Promega, Madison, WI, USA).
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