NETs were quantified using the Quanti-iT™ PicoGreen® dsDNA kit (Invitrogen, UK) and using a capture ELISA. Streptavidin-coated plates (Fisher Scientific, UK) were coated with an anti-MPO capture antibody (Abcam, UK) overnight at 4° C and blocked with 0.5% bovine serum albumin for 1 h at 37° C. Neutrophil supernatants were incubated for 2 h at 37° C. Further 1 h incubations were performed with an anti-citrullinated histone H3 detection antibody (Abcam, UK) and HRP-conjugated secondary antibody (Dako, UK). SureBlue TMB Microwell Peroxidase Substrate (KPL, UK) was then added and incubated in the dark at 37° C for 20 min and then stopped by the addition of TMB stop solution (KPL, UK). Absorbance was read at 450 nm using a Tecan GENios Spectra FLUOR plate reader (Tecan UK Ltd., UK).
Anti mpo capture antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-MPO capture antibody is a laboratory reagent designed to detect and quantify the presence of myeloperoxidase (MPO) in biological samples. This antibody binds specifically to MPO, which is an enzyme involved in the immune response and has been associated with various inflammatory conditions. The core function of this product is to provide a tool for researchers to measure MPO levels, supporting investigations into the role of MPO in disease processes and cellular mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Anti citrullinated histone h3 detection antibody, by Abcam (2 mentions)
Genios spectra fluor plate reader, by Tecan (2 mentions)
Quanti it picogreen dsdna kit, by Thermo Fisher Scientific (2 mentions)
Streptavidin coated plate, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated secondary antibody, by Agilent Technologies (2 mentions)
2 protocols using anti mpo capture antibody
1
Quantification of Neutrophil Extracellular Traps
2
Quantification of Neutrophil Extracellular Traps
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NETs was quantified using the Quanti-iT™ PicoGreen® dsDNA kit (Invitrogen, UK). Supernatants were also tested using a capture ELISA. Streptavidin-coated plates (Fisher Scientific, UK) were coated with an anti-MPO capture antibody (Abcam, UK) overnight at 4 o C and blocked with 0.5% bovine serum albumin for 1 hour at 37 o C. Neutrophil supernatants were incubated for 2 hours at 37 o C. Further 1 hour incubations were performed with an anticitrullinated histone H3 detection antibody (Abcam, UK) and HRP-conjugated secondary antibody (Dako, UK). SureBlue TMB Microwell Peroxidase Substrate (KPL, UK) was then added and incubated in the dark at 37 o C for 20 minutes and then stopped by the addition of TMB stop solution (KPL, UK). Absorbance was read at 450nm using a Tecan GENios Spectra FLUOR plate reader (Tecan UK Ltd., UK).
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