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Mercaptopurine

Manufactured by Merck Group
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Sourced in United States, Italy

Mercaptopurine is a laboratory reagent used for various research applications. It is a chemical compound that can be utilized in various experimental procedures. The core function of Mercaptopurine is to serve as a research tool, without further interpretation of its intended use.

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Lab products found in correlation

Doxycycline hydrochloride, by Merck Group (2 mentions) 6 thioguanine, by Merck Group (2 mentions) Itraconazole, by Merck Group (1 mentions) Fenpropimorph, by Merck Group (1 mentions) Fluconazole, by Merck Group (1 mentions) Cyclohexamide, by Merck Group (1 mentions) T butyl hydroperoxide, by Merck Group (1 mentions) Nano2, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Prednisolone, by Merck Group (1 mentions) Methotrexate, by Merck Group (1 mentions) Doxorubicin, by Cell Signaling Technology (1 mentions) Bortezomib, by Selleck Chemicals (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Everolimus, by Novartis (1 mentions) Lee011, by Novartis (1 mentions) Cisplatin, by Merck Group (1 mentions) Etoposide, by Merck Group (1 mentions)

6 protocols using mercaptopurine

1

Thiopurine Drugs in IHH Cell Viability

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IHH cells (1 x 10 6 ) were seeded in 25 cm 2 flasks and treated, for 48 and 96 hours, with azathioprine (1.4 x 10 -4 M, Sigma-Aldrich, Milan, Italy) and mercaptopurine (1.1 x 10 -3 M, Sigma-Aldrich, Milan, Italy) both diluted in NaOH 0.1 M. Control cultures were treated with the same volume of NaOH 0.1 M used for drug treatment (final concentration of NaOH 1.6 x 10 -4 M). The concentrations of azathioprine and mercaptopurine correspond to the IC50 [23] (link) obtained after a 96 hours thiopurine exposure previously evaluated in IHH cells by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, 5 mg/mL, Sigma-Aldrich, Milan, Italy) test.
Pelin M., Genova E., Fusco L., Marisat M., Hofmann U., Favretto D., Lucafò M., Taddio A., Martelossi S., Ventura A., Stocco G., Schwab M, & Decorti G. (2017). Pharmacokinetics and pharmacodynamics of thiopurines in an in vitro model of human hepatocytes: Insights from an innovative mass spectrometry assay. Chemico-biological interactions, 275.
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2

Comprehensive Phenotypic Assays for Yeast

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Phenotypic plate assays were performed on YNB (without amino acids and ammonium sulfate) media supplemented with 2% glucose and 10 mM ammonium sulfate unless stated otherwise. For UV sensitivity, cells were spotted then exposed for 6 sec to UV light at 48 mJ/cm2 in a UV Stratalinker (Stratagene, USA). For all other assays, the stressor was added to the media immediately prior to pouring at the following concentrations: 0.001% SDS (Sigma, USA), 5 μg/mL fluconazole (Sigma, USA), 0.25 μg/mL itraconazole (Sigma, USA), 1 μg/mL fenpropimorph (Sigma, USA), 1 M NaCl (Sigma, USA), 1 M KCl (Sigma, USA), 125 mM t-butyl hydroperoxide (Sigma, USA), 1 mM NaNO2 (Sigma, USA), 1 μg/mL cyclohexamide (Sigma, USA) and 1 mM mercaptopurine (Sigma, USA). Tenfold serial dilutions were prepared immediately prior to testing, with plates being imaged at 24–96 hr. All assays were performed at both 30 and 37 °C.
Arras S.D., Chitty J.L., Wizrah M.S., Erpf P.E., Schulz B.L., Tanurdzic M, & Fraser J.A. (2017). Sirtuins in the phylum Basidiomycota: A role in virulence in Cryptococcus neoformans. Scientific Reports, 7, 46567.
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3

Combination Therapy for Acute Leukemia

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LEE011 and everolimus were provided by Novartis. Methotrexate, mercaptopurine, dexamethasone and prednisolone were purchased from Sigma. Doxorubicin was purchased from Cell Signaling. Bortezomib was purchased from Selleckchem and Compound E was purchased from ENZO Pharmaceuticals. L-asparaginase was manufactured by Lundbeck Inc. and purchased from the Dana-Farber Cancer Institute pharmacy.
Pikman Y., Alexe G., Roti G., Conway A.S., Furman A., Lee E.S., Place A.E., Kim S., Saran C., Modiste R., Weinstock D.M., Harris M., Kung A.L., Silverman L.B, & Stegmaier K. (2016). Synergistic Drug Combinations with a CDK4/6 Inhibitor in T-cell Acute Lymphoblastic Leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(4), 1012-1024.
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4

Preparation of Anti-Cancer Drugs

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Cisplatin (Sigma-Aldrich), etoposide (Sigma-Aldrich), mercaptopurine (Sigma-Aldrich), and methotrexate (LKT Laboratories, St. Paul, MN, USA) were dissolved in dimethyl sulfoxide (DMSO) to generate 100 mM stock solutions.
Fukusumi H., Handa Y., Shofuda T, & Kanemura Y. (2018). Small-scale screening of anticancer drugs acting specifically on neural stem/progenitor cells derived from human-induced pluripotent stem cells using a time-course cytotoxicity test. PeerJ, 6, e4187.
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5

Biochemical Reagents and Antibodies

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Doxycycline hydrochloride (Sigma-Aldrich) was dissolved in MilliQ water. Thiopurines, 6-thioguanine (Sigma-Aldrich) and mercaptopurine (Merck AG), and all NUDT15 inhibitors were dissolved in DMSO to a stock of 10 mM. Antibodies against phosphorylated Chk1 (rabbit, Ser345; cat. no. 2348), Chk1 (mouse, cat. no. 2360), phosphorylated Chk2 (rabbit, Thr68; cat. no. 2197), Chk2 (mouse, cat. no. 3440), phospho-Histone H2A.X (rabbit, Ser139; cat. no. 2577), cleaved PARP (rabbit, cat. no. 9541), HA-tag (mouse, cat. no. 2367) and cleaved caspase 3 (rabbit, cat. no. 9661) were purchased from Cell Signaling Technology. Antibodies against NUDT15 (rabbit, cat. no. sc-84533), SOD1 (rabbit, FL-154; cat. no. sc-11407), phosphorylated CDK (rabbit, Thr14/Tyr15; cat. no. sc-28435-R) and Goat anti-mouse IgG-HRP secondary antibody (cat. no. sc-2055) were purchased from Santa Cruz Biotechnology, Inc.. Antibodies against GAPDH (rabbit, cat. no. ab9485) and β-Actin (mouse, cat. no. ab6276) were purchased from Abcam. Donkey anti-mouse IgG IRDye 680RD (cat. no. 925–68072) and goat anti-rabbit IgG IRDye 800CW (cat. no. 925–32211) were purchased from Li-Cor.
Zhang S.M., Desroses M., Hagenkort A., Valerie N.C., Rehling D., Carter M., Wallner O., Koolmeister T., Throup A., Jemth A.S., Almlöf I., Loseva O., Lundbäck T., Axelsson H., Regmi S., Sarno A., Krämer A., Pudelko L., Bräutigam L., Rasti A., Göttmann M., Wiita E., Kutzner J., Schaller T., Kalderén C., Cázares-Körner A., Page B.D., Krimpenfort R., Eshtad S., Altun M., Rudd S.G., Knapp S., Scobie M., Homan E.J., Berglund U.W., Stenmark P, & Helleday T. (2020). Development of a chemical probe against NUDT15. Nature chemical biology, 16(10), 1120-1128.
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6

Biochemical Reagents and Antibodies

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Doxycycline hydrochloride (Sigma-Aldrich) was dissolved in MilliQ water. Thiopurines, 6-thioguanine (Sigma-Aldrich) and mercaptopurine (Merck AG), and all NUDT15 inhibitors were dissolved in DMSO to a stock of 10 mM. Antibodies against phosphorylated Chk1 (rabbit, Ser345; cat. no. 2348), Chk1 (mouse, cat. no. 2360), phosphorylated Chk2 (rabbit, Thr68; cat. no. 2197), Chk2 (mouse, cat. no. 3440), phospho-Histone H2A.X (rabbit, Ser139; cat. no. 2577), cleaved PARP (rabbit, cat. no. 9541), HA-tag (mouse, cat. no. 2367) and cleaved caspase 3 (rabbit, cat. no. 9661) were purchased from Cell Signaling Technology. Antibodies against NUDT15 (rabbit, cat. no. sc-84533), SOD1 (rabbit, FL-154; cat. no. sc-11407), phosphorylated CDK (rabbit, Thr14/Tyr15; cat. no. sc-28435-R) and Goat anti-mouse IgG-HRP secondary antibody (cat. no. sc-2055) were purchased from Santa Cruz Biotechnology, Inc.. Antibodies against GAPDH (rabbit, cat. no. ab9485) and β-Actin (mouse, cat. no. ab6276) were purchased from Abcam. Donkey anti-mouse IgG IRDye 680RD (cat. no. 925–68072) and goat anti-rabbit IgG IRDye 800CW (cat. no. 925–32211) were purchased from Li-Cor.
Zhang S.M., Desroses M., Hagenkort A., Valerie N.C., Rehling D., Carter M., Wallner O., Koolmeister T., Throup A., Jemth A.S., Almlöf I., Loseva O., Lundbäck T., Axelsson H., Regmi S., Sarno A., Krämer A., Pudelko L., Bräutigam L., Rasti A., Göttmann M., Wiita E., Kutzner J., Schaller T., Kalderén C., Cázares-Körner A., Page B.D., Krimpenfort R., Eshtad S., Altun M., Rudd S.G., Knapp S., Scobie M., Homan E.J., Berglund U.W., Stenmark P, & Helleday T. (2020). Development of a chemical probe against NUDT15. Nature chemical biology, 16(10), 1120-1128.
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