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2 protocols using elvitegravir

1

Screening of Antiviral Compound Library

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Molecular biology grade DMSO was purchased from Sigma Aldrich (St. Louis, MO, USA), Sterile PBS was from ThermoFisher Scientific (Waltham, MA, USA). 3CLpro inhibitor screening enzymatic assay kits (Catalog #79955-1) were from BPS Biosciences (San Diego, CA, USA). Amprenavir, Atazanavir sulfate, Candicin, Chloroquine Phosphate, Hydroychloroquine Sulfate, and Lopinavir were purchased from Sigma Aldrich (Saint Louis, MO). Beclabuvir, Temsavir were from Medchem Express (Monmouth Junction, NJ). Abacavir (sulfate), Arbidol hydrochloride, Asunaprevir, Atrovastatin, Boceprevir, Daclatasvir, Danoprevir, Darunavir, Delavirdine (mesylate), Edoxudine, Elbasvir, Elvitegravir, Etravirine, Favipiravir, Fumagillin, Glecaprevir, Grazoprevir, Indinavir sulfate, Itraconazole, Ivermectin, Ledipasvir (G-5885), Maraviroc, Methylprednisolone, micafungin sodium, ombitasvir, Oseltamivir phosphate, Paritaprevir, Peramivir, Pibrentasvir, Pimodivir, Pleconaril, Posaconazole, Quinine, Raltegravir (potassium salt), Remdesivir, Ribavirin 5’-monophosphate (lithium salt), Ribostamycin sulfate, Rilpivirine, Saquinavir, Telaprevir, Tenofovir diphosphate (sodium salt), and Velpatasvir were purchased from Cayman Chemicals (Ann Arbor, MI).
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2

Antifolate Compound Characterization

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Dolutegravir and cabotegravir were provided by GlaxoSmithKline (Zebulon, NC). Bictegravir was purchased from Medkoo Biosciences (Morrisville, NC). Raltegravir and elvitegravir were obtained from Cayman Chemical (Ann Arbor, MI). Methotrexate, pemetrexed, valproic acid, and bromosulfophthalein were purchased from Sigma-Aldrich (St. Louis, MO). [ 3 H]-folic acid and [ 3 H]-Methotrexate were purchased from Moravek (Brea, CA). All other chemicals were of reagent grade and are readily available from commercial sources.
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