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Goat anti mouse il 15

Manufactured by R&D Systems

Goat anti-mouse IL-15 is a laboratory reagent that can be used to detect and quantify mouse interleukin-15 (IL-15) in various biological samples. It is a polyclonal antibody raised in goats against recombinant mouse IL-15.

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2 protocols using goat anti mouse il 15

1

Visualizing IL-15 Expression in Tumor Sections

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Tumors from TS/A cells infected with Ad.mIL-15, Ad.mIL-15 and Ad.mIL-15Rα or Ad.null each at an MOI of 100 were resected, fixed in OCT (SAKURA-Finetek, Torrance, CA) and sectioned by cryostat. Tissue sections were treated with 0.3% hydrogen peroxide, then treated with Avidin/Biotin blocking kit (Zymed, South San Francisco, CA) in accordance with the manufacturer’s instructions. The sections were stained with goat anti-mouse IL-15 (R &D systems). The slides were washed and stained using the anti-goat HRP-DAB Cell and Tissue staining kit (R&D Systems) as per the manufacturer’s instructions and counterstained with hematoxylin.
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2

Visualizing IL-15 Expression in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors from TS/A cells infected with Ad.mIL-15, Ad.mIL-15 and Ad.mIL-15Rα or Ad.null each at an MOI of 100 were resected, fixed in OCT (SAKURA-Finetek, Torrance, CA) and sectioned by cryostat. Tissue sections were treated with 0.3% hydrogen peroxide, then treated with Avidin/Biotin blocking kit (Zymed, South San Francisco, CA) in accordance with the manufacturer’s instructions. The sections were stained with goat anti-mouse IL-15 (R &D systems). The slides were washed and stained using the anti-goat HRP-DAB Cell and Tissue staining kit (R&D Systems) as per the manufacturer’s instructions and counterstained with hematoxylin.
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