Dulbecco s modified eagle

Manufactured by Lonza

Sourced in United States

Dulbecco's Modified Eagle Medium (DMEM) is a commonly used cell culture medium formulation. It provides the essential nutrients and components required to support the growth and maintenance of a wide range of mammalian cell lines in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin edta, by Merck Group (1 mentions) Raw264, by American Type Culture Collection (1 mentions) Dulbecco s phosphate, by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)

2 protocols using dulbecco s modified eagle

Culturing and Treating RAW264.7 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse macrophage cell line, RAW264.7 cells were purchased American Type Culture Collection (ATCC, Virginia, USA) and grown in Dulbecco's Modified Eagle (Lonza, Walkerscille, MD, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 ug/ ml streptomycin. The cells were cultured in an incubator containing 5% CO 2 at 37 °C. Trypsin-EDTA (Sigma, St. Louis, MO, USA) was used to detach the cells from a T75 flask. The novel compound was dissolved in dimethyl sulfoxide (DMSO) and treated to cells. DMSO was used as a vehicle and the final DMSO concentration did not exceed 0.1% (v/v).

Park G.H., Park Y., Hong S.S., & Park G.H. (2020). Anti-inflammatory effects of 18-nor-ent-pimara-9(11),15-diene-4β-ol isolated from the roots of Aralia continentalis on LPS-induced in RAW264.7 cells. Applied Biological Chemistry, 63(1).

+ Open protocol

+ Expand

Conditioning of Adipose-Derived Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

ADSCs (P6) were seeded into 225-cm 2 culture flasks (Corning, Inc., Corning, NY, USA) at a density of 1 × 10 4 cells/cm 2 in cultivation media. When reaching 80% confluence, cells were rinsed three times with Dulbecco's phosphate-buffered saline (DPBS; Gibco), and Dulbecco's modified Eagle's medium (DMEM; Lonza) was applied to the cells for 48-h conditioning at 37°C in a humidified atmosphere containing 10% CO 2 . At the end of incubation, the conditioned medium was harvested, centrifuged at 1,000 × g to remove debris and detached cells, concentrated 50-fold using Vivacell 70 (10 kDa; Sartorius, Goettingen, Germany), and stored at -80°C. All studies were performed with concentrated TFC.

Procházka V., Jurčíková J., Laššák O., Vítková K., Pavliska L., Porubová L., Buszman P.P., Krauze A., Fernandez C., Jalůvka F., Špačková I., Lochman I., Jana D., Merfeld-Clauss S., March K.L., Traktuev D.O, & Johnstone B.H. (2016). Therapeutic Potential of Adipose-Derived Therapeutic Factor Concentrate for Treating Critical Limb Ischemia. Cell transplantation, 25(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!