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Hydrogen peroxide

Manufactured by Sisco Research Laboratories
Sourced in India

Hydrogen peroxide is a clear, colorless liquid chemical compound with the formula H2O2. It has a wide range of industrial and commercial applications, including use as an oxidizing agent, disinfectant, and bleaching agent.

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5 protocols using hydrogen peroxide

1

Biochemical Assay Reagents and Protocols

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Thiobarbituric acid (TBA), bovine serum albumin (BSA), Sephadex G-25, and 5,5′ dithiobis 2-nitrobenzoic acid (DTNB) were purchased from Sigma Chemical Co., USA. NN′-bis-acrylamide and NNN′N′-tetramethylethylenediamine (TEMED) were purchased from Merk-Schudart, and reduced glutathione, ascorbic acid, horse radish peroxidase, hydrogen peroxide, 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, acrylamide, sodium dodecyl sulfate (SDS), ferric chloride, potassium ferricyanide, and ammonium persulfate were obtained from SISCO Research Laboratory, India. Digitonin was procured from Hi-media Ltd., India. All other chemicals used were of analytical grade.
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2

Graphene Fabrication from Expandable Graphite

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Expandable graphite (EG, 99.9%) purchased from Asbury Carbons (USA) was used for fabrication of graphene. The chemical reagents such as sodium borohydride (NaBH4) (>99% purity), hydrogen peroxide (30%), ferric (III) chloride (>96%), 4-nitrophenol (4-NP), sodium hydroxide (NaOH) (>97%), sodium hypochlorite (4%), and sulphuric acid (>99%), (Analytical Grade (AR)) used in the study were obtained from SISCO Research laboratories (SRL Chemicals, India). Bamboo pulp, was selected as the initial cellulosic precursor for the fabrication of CNCs, which was received from Hindustan Paper Corporation Limited (HPCL, Assam, India). The platinum (PtCl2) (99.9%) and palladium chloride (PdCl2) (99%) salts used in this study were purchased from Sigma Aldrich, India.
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3

Synthesis and Characterization of Iron Complexes

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Fe(NO3)3·9H2O, FeCl3·3H2O, and Fe(C2H3O2)2 were purchased from TCI chemicals.
Poly(ethylene glycol) 200 (PEG-200), methyl orange (MO), rhodamine
B (RhB), methylene blue (MB), and terephthalic acid were obtained
from Sigma-Aldrich. 3,3,5,5′-Tetramethylbenzidine (TMB), 1,2,3-trihyroxybenzene
(Pyrogallol), and hydrogen peroxide (30%) were obtained from Sisco
Research Laboratories (SRL), India. Sodium acetate trihydrate, acetic
acid, and NaH2PO4 were purchased from Merck
Ltd, India. Ultra-pure water obtained from the Milli-Q system was
used in all experiments.
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4

Assay of Antioxidant and Enzymatic Activities

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Pyrogallol, Catalase, reduced glutathione (GSH), 2,2- diphenyl-1-picrylhydrazyl (DPPH), 1-chloro-2,4-dinitrobenzene (CDNB), Acetylthiocholine chloride, and 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) were procured from Sigma Chemical Co. (St. Louis, MO, USA); hydrogen peroxide, sodium hydroxide, sodium di-hydrogen phosphate, L-ascorbic acid, and sodium carbonate were obtained from Sisco Research Laboratory, Mumbai, India. Sodium Chloride, Magnesium Chloride, and Tris-base were purchased from Himedia Laboratories, Mumbai.
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5

Evaluation of Antioxidant and Hepatoprotective Potential

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Silymarin (Sigma), ellagic acid (Himedia laboratories Pvt Ltd.), diclofenac sodium (Novartis, India), ABTS (Sisco Research Laboratory Pvt. Ltd.) were procured from standard suppliers. Biochemical kits for estimation of creatinine, uric acid, total protein, alkaline phosphatase (ALP), serum glutamic-oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), γ-glutamyl transferase (GGT) and lactate dehydrogenase (LDH) were purchased from Erba Transasia Bio-medicals Ltd. All other laboratory chemicals such as 2,4,6-tripyridyl-s-triazine (TPTZ), potassium persulfate, bovine serum albumin, sodium hydroxide, hydrogen peroxide etc. were purchased from Sisco research laboratory Pvt. Ltd. New Delhi, India.
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