Gas chromatography–mass spectrometry (GC–MS) combined with solid phase microextraction in the gas phase (SPME Arrow, Arrow fiber, Shimadzu, Kyoto, Japan) was used for the separation, identification, and quantification of volatile organic compounds (VOC). VOCs were performed with Shimadzu GCMS QP 2020 Plus (Shimadzu, Kyoto, Japan) equipped with a ZB-5Msi capillary column (30 m × 0.25 mm × 0.25 µm; Phenomenex Ltd., Torrance, CA, USA).
The operational conditions of the GC were as follows: injection port 50 °C; temperature program started at 50 °C and held for 2 min, then at the of rate 3 °C·min−1 to 180 °C, then at the rate of 20 °C·min−1 to 270 °C, held for 5 min, 10 s; helium as carrier gas with flow 1 mL·min−1; split 100 (SPME Arrow analysis).
The extraction of VOCs was performed with 1.10 mm DVB/C-WR/PDMS SPME Arrow fibre (Supelco, Bellefonte, PA, USA). Extraction was carried out in 20 mL headspace vials for 30 min at 45 °C. The extraction proceeded with incubation for 10 min at the same temperature. The analytes were desorbed under the conditions of the GC injection port for 3 min. MS operational conditions were as follows: interface temperature 250 °C; ion source temperature 250 °C; scan 40–400 m/z.
The operational conditions of the GC were as follows: injection port 50 °C; temperature program started at 50 °C and held for 2 min, then at the of rate 3 °C·min−1 to 180 °C, then at the rate of 20 °C·min−1 to 270 °C, held for 5 min, 10 s; helium as carrier gas with flow 1 mL·min−1; split 100 (SPME Arrow analysis).
The extraction of VOCs was performed with 1.10 mm DVB/C-WR/PDMS SPME Arrow fibre (Supelco, Bellefonte, PA, USA). Extraction was carried out in 20 mL headspace vials for 30 min at 45 °C. The extraction proceeded with incubation for 10 min at the same temperature. The analytes were desorbed under the conditions of the GC injection port for 3 min. MS operational conditions were as follows: interface temperature 250 °C; ion source temperature 250 °C; scan 40–400 m/z.