Hct 8 cells

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

HCT-8 cells are a human colon adenocarcinoma cell line. They are a commonly used in vitro model for the study of intestinal physiology and pathology.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

HCT-8 (9 citations)

7 protocols using hct 8 cells

Cryptosporidium parvum Oocyst Purification and Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cryptosporidium parvum oocysts (IOWA isolate) were purchased from Waterborne, Inc. (New Orleans, LA, United States) and stored in phosphate-buffered saline (PBS) with antibiotics at 4°C. All oocysts used in this study were stored for less than 3 months. Before usage, oocysts were treated on ice with chilled 0.5% sodium hypochlorite for 10 min and washed three times afterward with PBS by centrifugation at 13,200 × g for 2 min.
Human colon adenocarcinoma cells (HCT-8 cells) were purchased from the cell bank of the Chinese Academy of Sciences. They were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C and 5% CO₂.

Zhang Q., Guo Y., Li N., Li Y., Su J., Xu R., Zhang Z., Feng Y, & Xiao L. (2020). Characterization of Calcium-Dependent Protein Kinases 3, a Protein Involved in Growth of Cryptosporidium parvum. Frontiers in Microbiology, 11, 907.

+ Open protocol

+ Expand

Culturing Human Colon Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colon cancer HCT-8 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in Dulbecco's modified Eagle's medium (Santa Clara, State of California) containing 10% fetal calf serum, 100 μg/mL penicillin, and 100 μg/mL streptomycin, at 37°C in a CO 2 incubator. The cells were subcultured every 2-3 days, after treatment with 0.02% ethylenediaminetetraacetic acid and 0.1% trypsin.

Han Z., Huang H, & Zhang T. (2019). Downregulation of DBN1 is related to vincristine resistance in colon cancer cells. Journal of cancer research and therapeutics, 15(1).

+ Open protocol

+ Expand

Colon Cancer Cell Line Culture and STAT5 Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HCT116, SW620, and RKO COAD cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). HCT8 cells were obtained from the China Center for Type Culture Collection (Wuhan, China). The NCM460 cell line was purchased from INCELL (San Antonio, TX, USA). The SW620 and HCT116 cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA), RKO cells were cultured in Minimum Essential Medium (Gibco), HCT-8 cells were cultured in RPMI-1640 (Gibco), and NCM460 cells were cultured in M300F (INCELL). All cell culture media were supplemented with 10% fetal bovine serum (Gibco) and penicillin/streptomycin (Hyclone, Logan, UT, USA), and the cells were cultured in a humidified incubator at 37°C and 5% CO₂.
The STAT5 inhibitor pimozide was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and used to treat the colon cancer cells at a dose of 10 μM for 24 h.

Chen G., Lian D., Zhao L., Wang Z., Wuyun Q, & Zhang N. (2022). The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis. Bioengineered, 13(4), 11083-11095.

+ Open protocol

+ Expand

Propagation of Attenuated PRV Vaccine Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colon cancer cell line HCT-8 cells were purchased from China Center for Type Culture Collection and cultured in RPMI 1640 medium (Gibco, Carlsbad, CA, USA) containing 10% FBS (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin-streptomycin. VERO (African green monkey kidney) cells were purchased from the China Center for Type Culture Collection and cultured in DMEM medium (Gibco, Carlsbad, CA, USA) containing 10% FBS. PRV Bartha K61 strain and PRV HB98 strain are widely used live attenuated vaccine strains, which were purchased from China Animal Husbandry Industry Co., Ltd., (Beijing, China) and Wuhan Keqian Animal Biological Products Co., Ltd., (Wuhan, China) respectively. The PRV Bartha K61 strain lacks primarily the gE and gI genes, whereas the PRV HB98 strain lacks the TK, gG, and gE genes [22 (link),23 (link)]. The viruses were propagated in VERO cells and stored at −70 °C until use.

Chai C., Zhang J., Zhou Y., Yin H., Zhang F., Diao Y., Zan X., Ma Y., Wang Y., Wu Y, & Wang W. (2022). The Effects of Oncolytic Pseudorabies Virus Vaccine Strain Inhibited the Growth of Colorectal Cancer HCT-8 Cells In Vitro and In Vivo. Animals : an Open Access Journal from MDPI, 12(18), 2416.

+ Open protocol

+ Expand

Fecal Virus Isolation and Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following positive PCR results, the fecal supernatant was filtered with a 0.45 μm filter and treated with 10 µg/mL trypsin (Gibco, USA) for 30 min, then the treated fecal supernatant was inoculated for 1 h. HCT-8 cells, obtained from the China Center for Type Culture Collection, were cultured at 37℃ with 5% CO₂ in RPMI 1640 containing 5 µg/mL trypsin for 3 days after the inoculum was discarded. After observing CPE (cytopathic effect), the virus was collected.

Zhang F., Chai C., Niu R., Diao Y., Zhou Y., Zhang J., Feng L., Yao C., Wu Y., Ma Y., Zan X, & Wang W. (2024). Genetic characterization of bovine coronavirus strain isolated in Inner Mongolia of China. BMC Veterinary Research, 20, 209.

+ Open protocol

+ Expand

HCT-8 Cell Culture Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

HCT-8 cells (Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China) were maintained (37°C, 5% CO₂) in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 µg/ml penicillin, and 100 µg/ml streptomycin. The cells were passaged every 2–3 days, using 0.02% EDTA and 0.1% trypsin.

Han Z.M., Huang H.M, & Sun Y.W. (2018). Effect of CEACAM-1 knockdown in human colorectal cancer cells. Oncology Letters, 16(2), 1622-1626.

+ Open protocol

+ Expand

Establishing Multidrug-Resistant Colorectal Cancer Cell Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colorectal cancer cells HCT116 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China); The multidrug-resistant human colorectal cancer cell line HCT-8/5-FU (MDR) was developed by incubating parental HCT-8 cells (TCCCAS, Shanghai, China) according to the method described by Han et al [36 (link)]. As a non-cancerous counterpart, human colonic epithelial NCM460 cells were purchased from INCELL, San Antonio, USA. Cells were maintained in RPMI-1640 medium which were supplemented with 10% (v/v) fetal calf serum, 1% (v/v) nonessential amino acids, 1% (v/v) sodium pyruvate, 12 mM L-glutamine, and 1% (v/v) penicillin/streptomycin (all from Invitrogen). at 37°C in a water-saturated atmosphere with 5% CO₂, and subcultured by standard methods.

Yu N., Zhu H., Yang Y., Tao Y., Tan F., Pei Q., Zhou Y., Song X., Tan Q, & Pei H. (2017). Combination of Fe/Cu –chelators and docosahexaenoic acid: an exploration for the treatment of colorectal cancer. Oncotarget, 8(31), 51478-51491.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!