Mouse anti gapdh

Protein samples were separated by 10% SDS-PAGE and electroblotted onto PVDF membranes (Roche). After blocking, the blots were then incubated with monoclonal or polyclonal primary antibodies (mAb or pAb), followed by secondary IRDye® 680RD goat anti-mouse (1:5000) or goat anti-rabbit (1:5000) antibody (LI-COR Biosciences, Lincoln, NE, USA). Rabbit anti- Na_v1.7 (1:500) and anti-Na_v1.8 (1:500) pAbs were purchased from Alomone Labs (Jerusalem, Israel); mouse anti-p50 (1:200), anti-p65 (1:200) and anti-p-p50 (1:500) mAbs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit anti-p-p65 (1:500) mAb were purchased from Cell Signaling Technology (Danvers, MA, USA); mouse anti-ADRB2/β₂AR (1:1000) mAb and rabbit anti-p-ADRB2/β₂AR (1:500) pAb were purchased from Proteintech (Wuhan, Hubei, China) and Sigma-Aldrich (USA), respectively; mouse anti-GAPDH (1:1000) and anti-SMN (1:1000) mAbs were purchased from MultiSciences (Hangzhou, Zhejiang, China) and BD Biosciences (San Diego, CA, USA), respectively. Protein signals were analyzed with an Odyssey Infrared Imaging System (LI-COR, Lincoln, NE, USA).