Purified anti-mouse CD3 (145-2C11) and anti-mouse CD28 (37.51) mAbs and all the antibodies used in flow cytometry were purchased from BD PharMingen (San Diego, CA). Protein G-Sepharose was purchased from GE Healthcare (Piscataway, NJ). Anti-Cbl-b (G-1), anti-HA (D-8 and Y-11), anti-His (H-3), anti-Flag (D-8), anti-Stub1 (G-2), anti-c-Cbl (A-9), anti-Cul2 (H-300), and anti-ubiquitin (P4D1) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated goat anti-rabbit IgG or rabbit anti-mouse IgG were purchased from Kirkegaard & Perry Laboratories (Gaithersburg, MD).
Purified anti mouse cd3 145 2c11
Manufactured by BD
Sourced in United States
Purified anti-mouse CD3 (145-2C11) is a laboratory reagent used for the detection and analysis of mouse T cells. It binds to the CD3 complex, a key component of the T cell receptor, and can be used for the identification and isolation of mouse T cells in various applications.
Automatically generated - may contain errors
2 protocols using purified anti mouse cd3 145 2c11
1
Antibody-mediated T-cell activation
2
Multiparametric Flow Cytometry Analysis
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FITC-conjugated anti-mouse CD8 (53-6.7), PerCP-cy5.5-conjugated anti-mouse CD4 (RM4-5), PE-conjugated anti-mouse CD25 (3C7), APC-conjugated anti-mouse CD69 (H1.2F3), APC-conjugated anti-mouse CD3 (145-2C11), APC-cy7-conjugated anti-mouse CD3 (145-2C11), PE-conjugated anti-mouse TLR4 (MTS510), APC-conjugated anti-mouse IFN-γ (XMG1.2), PE-conjugated anti-mouse IL-4 (11B11), and APC-conjugated anti-mouse TLR3 (11F8) were purchased from BD Pharmingen (San Diego, CA, USA). FITC-conjugated anti-mouse TLR2 (T2.5) and FITC-conjugated anti-mouse CD127 (ATR34) were purchased from Bio-Legend (San Diego, CA, USA). Purified anti-mouse CD3 (145-2C11) and anti-mouse CD28 (37.51) were purchased from BD Pharmingen (San Diego, CA, USA).
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