10-color Gallios B43618 (Beckman Coulter, Indianapolis, IN) and 14-color Attune NxT (ThermoFisher Scientific) were used to acquire data. Analysis was performed with FlowJo software. Cells were counted with 123count eBeads (Thermo Fisher). Expression of CAR was detected by myc tag (9E10, Alexa Fluor 647, ThermoFisher) or violet-excitable GFP tag. DAPI (0.5 mg/ml, Sigma-Aldrich) or a LIVE/DEAD fixable yellow fluorescent dye (Thermo Fisher) were used to exclude dead cells in all experiments. Sorting of splenocytes after tissue processing was done using a BD FACSAria under sterile conditions. Antibodes to the following mouse proteins were used for flow cytometry: CD3ε (145-2C11,eBioscience, cat# 11-0031-85), CD3 (17A2, BioLegend, cat# 100233), CD4 (GK1.5, eBioscience, cat# 48-0041-80), CD4 (RM4-5, eBioscience, cat# 69-0042-80), CD8α (53-6.7, eBioscience, cat# 48-0041-80), CD19 (eBio1D3, eBioscience, cat# 48-0193-82), CD25 (PC61.5, eBioscience, cat# 25-0251-81), CD45 (30-F11, BioLegend, cat# 103106), CD80 (16-10A1, eBioscience, cat# 46-0801), CD86 (GL1, eBioscience, cat# 25-0862), CD223LAG-3 (C9B7W, eBioscience, cat# 12-5956-80), CD279 PD-1 (J43, eBiosceince, cat# 48-9985-82), and TIM3(8B.2C12, eBiosceince, cat# 48-5871-80).
C9b7w
Manufactured by Thermo Fisher Scientific
Sourced in United States
The C9B7W is a laboratory instrument designed for a specific purpose. It is a compact and reliable tool used in various research and analytical applications. The core function of the C9B7W is to perform a particular task with precision and accuracy. Further details about its intended use or performance specifications are not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Attune nxt, by Thermo Fisher Scientific (2 mentions)
Rm4 5, by Thermo Fisher Scientific (1 mentions)
Ebio1d3, by Thermo Fisher Scientific (1 mentions)
Gallios b43618, by Beckman Coulter (1 mentions)
123count ebeads, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Facsaria, by BD (1 mentions)
Fjk 16, by Thermo Fisher Scientific (1 mentions)
Jes6 5h4, by BD (1 mentions)
Bcl 10c4, by BioLegend (1 mentions)
Tc11 18h10, by BD (1 mentions)
Bcl 2, by BioLegend (1 mentions)
Rm4 5, by BD (1 mentions)
Lag 3, by Thermo Fisher Scientific (1 mentions)
Cd103, by BD (1 mentions)
Klrg1, by Thermo Fisher Scientific (1 mentions)
Uc10 4f10 11, by BD (1 mentions)
Ngfr5, by Thermo Fisher Scientific (1 mentions)
Mp1 22e9, by BD (1 mentions)
Jes5 16e3, by BD (1 mentions)
3 protocols using c9b7w
1
Multicolor Flow Cytometry Analysis of CAR T Cells
2
LAG-3 Expression Quantification
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3A9-mLAG-3 cells were incubated at 1.5 × 105 cells/well with anti-mLAG3 antibody C9B7W (eBioscience) or controls, and PE-labeled OVA323-339 MHC-II-IAb tetramers (MBL International, USA). After 60 min at 4°C, cells were washed and analyzed by flow cytometry.
3
Flow Cytometry Antibody Panel for Immune Cell Profiling
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The following antibodies were used for the flow cytometry analysis and cell sorting: CD4 (1:400, RM4-5, #553051), CD8 (1:400, 53-6.7, #553033), CD44 (1:400, IM7, #553134), CD25 (1:200, PC61, #562606), IL-2 (1:200, JES6-5H4, #554428), IL-4 (1:200, 11B11, #554435), IL-10 (1:200, JES5-16E3, #554467), IL-17 (1:200, TC11-18H10, #560184), GM-CSF (1:200, MP1-22E9, #564747), PD-1 (1:200, J43, #562584), CTLA-4 (1:200, UC10-4F10-11, #553720), and GITR (1:200, DTA-1, #558140) antibodies were purchased from BD Biosciences. Ki67 (1:100, anti-human, clone B56, #556027), CD103 (1:100, M290, #557495), and ICOS (1:200, 7E.17G9, #552146) antibodies were purchased from BD Pharmingen. IFN-γ (1:200, XMG1.2, #25-7311-41), Foxp3 (1:200, FJK-16s, #12-5773-82), LAG3 (1:200, C9B7W, #17-2231-82), Tim3 (1:200, RMT3-23, #12-5870-82), and KLRG1 (1:200, 2F1, #25-5893-82) antibodies were purchased from eBioscience. TIGIT (1:200, 1G9, #142103) and Bcl2 (1:100, BCL/10C4, #633503) antibodies were purchased from Biolegend. NGFR (1:800, NGFR5, #MS-394-B1) and Live/Dead cell stain kit (#L34955) were purchased from Thermo Fisher Scientific.
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