Ammonium formate, methanol, phosphoric acid, sodium dodecyl sulfate (SDS) and sodium hydroxide were obtained from Fisher Scientific (Fair Lawn, NJ). Cotinine was purchased from Alfa Aesar (Ward Hill, MA) and a stock solution (1 mg/mL) was prepared in methanol and stored at −20°C. The micellar background electrolyte (BGE) contained 50 mM phosphoric acid and 75 mM SDS (pH 2.5). The non-micellar BGE was 50 mM phosphoric buffer (pH 2.5). The high-conductivity buffer (HCB) was 75 mM phosphoric buffer (pH 2.5). The sample matrix solution for CE analysis was 0.1 mM phosphoric acid. Purified water from a Millipore Nanopure water system (Barnstead, Dubuque, IA) was used to prepare all solutions.
Cotinine
Manufactured by Thermo Fisher Scientific
Sourced in Morocco, Germany
Cotinine is a chemical compound that is commonly used as a biomarker to detect and measure exposure to nicotine. It is the primary metabolite of nicotine and can be detected in various biological samples such as blood, urine, or saliva. Cotinine analysis is often used to assess tobacco or nicotine use, exposure, and metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (2 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Phosphoric acid, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Anti gapdh antibody, by Merck Group (1 mentions)
Anti sod1, by Santa Cruz Biotechnology (1 mentions)
Anti phospho nrf2 antibody, by Abcam (1 mentions)
Sc 11407, by Santa Cruz Biotechnology (1 mentions)
Sc 23950, by Santa Cruz Biotechnology (1 mentions)
Anti phospho p38 map kinase, by Cell Signaling Technology (1 mentions)
N acetylcysteine nac, by Carl Roth (1 mentions)
Anti acetylated α tubulin, by Santa Cruz Biotechnology (1 mentions)
Sc 2004, by Santa Cruz Biotechnology (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
2 protocols using cotinine
1
Capillary Electrophoresis of Cotinine
2
Antibody Sourcing and Chemical Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-acetylated-α-tubulin, anti-SOD-1, and anti-rabbit HRP-labeled secondary antibodies were obtained from Santa Cruz (SC-23950, SC-11407, and SC-2004; Heidelberg, Germany). Anti-GAPDH antibody was obtained from Sigma-Aldrich (G9545; Munich, Germany). Anti-phospho-p38 MAPKinase and anti-catalase antibodies were obtained from Cell Signaling (Frankfurt am Main, Germany). Anti-phospho-Nrf2 antibody was obtained from Abcam (ab76026; Cambridge, United Kingdom). Alexa Fluor 448-labeled secondary antibody was obtained from Invitrogen (Karlsruhe, Germany). N-Acetylcysteine (NAC) and nicotine were obtained from Carl Roth (Karlsruhe, Germany). L-Ascorbic acid was obtained from Sigma-Aldrich (Darmstadt, Germany). Cotinine was obtained from Alfa Aesar (Karlsruhe, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!