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Potassium phosphate monobasic

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Potassium phosphate monobasic is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that is soluble in water. The core function of potassium phosphate monobasic is to serve as a buffering agent, maintaining pH levels in various laboratory applications.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (4 mentions) Potassium chloride (kcl), by Avantor (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Sodium dodecyl sulfate (sds), by Avantor (1 mentions) Nupage antioxidant, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Nupage sample reducing agent, by Thermo Fisher Scientific (1 mentions) Pre stained protein standard, by Thermo Fisher Scientific (1 mentions) Bromophenol blue, by Avantor (1 mentions) Coomassie blue r 250, by Avantor (1 mentions) Nupage mes sds running buffer, by Thermo Fisher Scientific (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Silver wire, by Thermo Fisher Scientific (1 mentions) Methotrexate, by MedChemExpress (1 mentions) Hplc grade solvents, by Thermo Fisher Scientific (1 mentions) Potassium phosphate dibasic, by Merck Group (1 mentions) Imidazole im, by Thermo Fisher Scientific (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonicacid hepes, by GoldBio (1 mentions)

Close spelling variants of this product

Potassium phosphate (7 citations) Potassium phosphate monobasic (KH2PO4) (4 citations)

12 protocols using potassium phosphate monobasic

1

Comprehensive Protein Quantification Protocol

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Unless otherwise specified, all reagents were purchased from Millipore-Sigma (Oakville, ON, Canada). The BCA protein assay reagent (bicinchoninic acid), NuPAGE® 4–12% Bis-Tris gels, NuPAGE® MES SDS running buffer, NuPAGE® sample reducing agent, NuPAGE® antioxidant, NuPAGE® LDS sample buffer, prestained protein standard, methanol, sodium chloride, and Corning clear polystyrene 96-well microplates were purchased from ThermoFisher Scientific (Nepean, ON). Glacial acetic acid, glycerol, sodium phosphate dibasic, and trizma base were from Bioshop Canada (Burlington, ON, Canada). Bromophenol blue, Coomassie blue R250, potassium chloride, potassium phosphate monobasic, and sodium dodecyl sulfate were from VWR (Mississauga, ON, Canada). Petri dishes (150 mm) were purchased from Ultident Scientific (St. Laurent, QC, Canada).
Ahmed T.A., Cordeiro C.M., Elebute O, & Hincke M.T. (2022). Proteomic Analysis of Chicken Chorioallantoic Membrane (CAM) during Embryonic Development Provides Functional Insight. BioMed Research International, 2022, 7813921.
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2

Human Skin-Based Methotrexate Electroanalysis

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Methotrexate was purchased from MedChemExpress LLC (NJ, USA), Sodium phosphate dibasic heptahydrate, and potassium phosphate monobasic were obtained from VWR (Atlanta, GA, USA) and Thermo Fisher Scientific (Waltham, MA, USA), respectively. Silver wire (0.5 mm diameter, 99.99%) was obtained from Fisher Scientific (Fair Lawn, NJ, USA), and silver/silver chloride electrodes (2 mm × 4 mm) were obtained from A-M systems (Sequim, WA, USA). All HPLC grade solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA).
Fresh frozen full-thickness human (healthy and diseased) cadaver skin from multiple sites (abdomen, ankle, and calf) from different donors was obtained from National Disease Research Interchange. De-identified human skin was used under a protocol exempted by Mercer University Institutional Review Board (H0303041).
Vora D., Garimella H.T., German C.L, & Banga A.K. (2022). Microneedle and Iontophoresis Mediated Delivery of Methotrexate into and across Healthy and Psoriatic Skin. International journal of pharmaceutics, 618, 121693.
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3

Preparation of Experimental Buffers

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All reagents were used as purchased, unless specified. Buffers used in this study were prepared using deionized water (18.2 MΩ, Elga Technologies), and the pH was adjusted accordingly using KOH or HCl to achieve a final pH of 7.0 for all experiments. The following buffers were used: sodium bicarbonate (Sigma-Aldrich), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; GoldBio), imidazole (Im; Alfa Aesar‡), 3-(N-morpholino)propanesulfonic acid (MOPS; GoldBio), potassium phosphate dibasic (Sigma-Aldrich), potassium phosphate monobasic (VWR), piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES; BDH), triethanolamine (TEOA; J.T Baker), and tris(hydroxymethyl)aminomethane (Tris; VWR).
Schneider C.R., Lewis L.C, & Shafaat H.S. (2019). The Good, the Neutral, and the Positive: Buffer Identity Impacts CO2 Reduction Activity by Nickel(II) Cyclam. Dalton transactions (Cambridge, England : 2003), 48(42), 15810-15821.
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4

Formulation and Characterization of Lipid-Based Drug Delivery Systems

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Capmul MCM (HLB 4.7) was obtained as a gift from Abitec Corporation (Columbus, OH). The composition of Capmul MCM is approximately 60% of medium-chain monoglycerides and 40% of diglycerides, derived from caprylic acid (83%) and capric acid (17%). Danazol was donated by Sanofi-Aventis Pharmaceutics (Bridgewater, NJ). Caprylic acid (HLB 1), indomethacin, haloperidol, hydrochloric acid (1 N), potassium chloride, potassium phosphate monobasic, sodium hydroxide, sodium acetate, acetic acid, and polyethylene glycol (PEG) 400 were purchased from VWR (Solon, OH). Cremophor RH40 (HLB 14–16) was supplied by BASF Corporation (Tarrytown, NJ). All chemicals and solvents were of analytical purity or high performance liquid chromatography (HPLC) grade and used as received with no further treatment.
Li S., Madan P, & Lin S. (2016). Effect of ionization of drug on drug solubilization in SMEDDS prepared using Capmul MCM and caprylic acid. Asian Journal of Pharmaceutical Sciences, 12(1), 73-82.
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5

Pharmacological Protein Purification Protocol

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Sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), and acetonitrile were all purchased from VWR International (Ville Mont-Royal, QC, Canada). Anti-polyethylene glycol antibody [PEG-B-47] was obtained from Abcam (Toronto, ON, Canada). SureBeads magnetic beads with protein A were bought from Biorad Laboratories (Mississauga, ON, Canada). Tween-20 was purchased from Fisher Scientific (Ottawa, ON, Canada). Flurbiprofen and ketorolac were obtained from Cayman Chemical Company (Ann Harbour, MI, USA).
Raiche-Marcoux G., Loiseau A., Maranda C., Poliquin A, & Boisselier E. (2022). Parametric Drug Release Optimization of Anti-Inflammatory Drugs by Gold Nanoparticles for Topically Applied Ocular Therapy. International Journal of Molecular Sciences, 23(24), 16191.
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6

Synthesis and Characterization of FITC-BSA

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Ethylene glycol, phosphate-buffered saline (PBS, pH 7.4), fluorescein isothiocyanate bovine serum albumin (FITC-BSA), TMA, CAA, TEGDMA, DEGDMA, Tetra-EGDMA, sodium hydroxide, ammonium persulfate (APS), sodium metabisulfate (SMS), N-(3dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich. Potassium phosphate monobasic and sodium phosphate dibasic, anhydrous were purchased from VWR. Ethanol was purchased from Greenfield Global. All chemicals were used as received.
Mariner E., Haag S.L, & Bernards M.T. (2019). Impacts of cross-linker chain length on the physical properties of polyampholyte hydrogels. Biointerphases, 14(3).
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7

Proteomic Analysis of Oxidative Stress

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The reagents used in this study included AAPH (EMD Chemicals, Gibbstown, NJ, USA), tris (2-carboxyethyl) phosphine (TCEP), trifluoroacetic acid (TFA), formic acid (Thermo Scientific, Waltham, MA, USA), DL-dithiothreitol (DTT), iodoacetic acid (IAA), L-Met, L-Trp, L-Tyr, pyridoxine (vitamin B6) (Sigma-Aldrich, St. Louis, MO, USA), trypsin (Roche, Indianapolis, IN, USA), water, acetonitrile, potassium phosphate monobasic, potassium phosphate dibasic (J.T. Baker, Phillipsburg, NJ, USA), and potassium chloride (Mallinckrodt Chemicals, Phillipsburg, NJ, USA).
Zheng K., Ren D., Wang Y.J., Lilyestrom W., Scherer T., Hong J.K, & Ji J.A. (2021). Monoclonal Antibody Aggregation Associated with Free Radical Induced Oxidation. International Journal of Molecular Sciences, 22(8), 3952.
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8

Quantifying Mycophenolic Acid Metabolites

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MPA, MPA phenol glucuronide, MPA phenol glucuronide-D3, MPA acyl glucuronide, and MPA phenol glucoside were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). The cofactors uridine 5’-diphosphoglucose disodium salt (UDP-glucose) and uridine 5’-diphosphoglucuronic acid disodium salt (UDP-glucuronic acid) and alamethicin a peptide that forms pores in lipid bilayer membranes to enhance co-factor availability, were purchased from Sigma-Aldrich (St. Louis, MO). Potassium phosphate monobasic and magnesium chloride were purchased from JT Baker (Austin, TX). Acetonitrile, acetic acid, formic acid and ammonia solution were purchased from Fisher Scientific (Pittsburgh, PA).
Slovak J., Mealey K, & Court M. (2016). Comparative metabolism of mycophenolic acid by glucuronic acid and glucose conjugation in human, dog, and cat liver microsomes. Journal of veterinary pharmacology and therapeutics, 40(2), 123-129.
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9

Diazoxide Oral Formulation Development

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Diazoxide, glycerin, 50 mL amber PET bottles with black phenolic caps, 1 mL amber plastic oral syringes with tip caps, Oral Mix and Oral Mix SF vehicles used in this study were provided by Medisca Pharmaceutique Inc. (QC, Canada). All chemicals used are at United States Pharmacopeia standards when applicable. Diazoxide 100 mg capsules (Proglycem, Merck, Canada) were also used. Acetonitrile and dimethylsulfoxide were HPLC grade and were obtained from Fisher Scientific (QC, Canada). Potassium phosphate monobasic was purchased from JT Baker (NJ, USA). Purified water was deionised using a MilliQ system to 18 MΩ resistivity.
Friciu M., Zaraa S., Roullin V.G, & Leclair G. (2016). Stability of Diazoxide in Extemporaneously Compounded Oral Suspensions. PLoS ONE, 11(10), e0164577.
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10

Preparation of Films and Simulated Saliva Medium

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For the preparation of the films, LC was obtained from Maver. GG and Propylene Glycol were purchased from Cosmopolita Drugstore (Mexico). PVP (Kollidon 30) was acquired from BASF (USA).
For the preparation of the Simulated Saliva Medium (SSM), citric acid was obtained from Barsa. Dibasic Sodium Phosphate and Monobasic Potassium Phosphate were acquired from J.T.Baker (USA). Calcium Carbonate was purchased from Paris Drugstore (Mexico). Calcium Chloride, Potassium Chloride, and Sodium Chloride were acquired from Cosmopolita Drugstore (Mexico).
Robles-Kanafany C.M., Del Prado-Audelo M.A.L., González-Torres M., Giraldo-Gomez D.M., Caballero-Florán I.H., González-Del Carmen M., Sharifi-Rad J., Figueroa-González G., Reyes-Hernández O.D., Cortés H, & Leyva-Gómez G. (2021). Development of a guar gum film with lysine clonixinate for periodontal treatments. Cellular and molecular biology (Noisy-le-Grand, France), 67(1).
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